Ghrelin in the lateral parabrachial nucleus influences the excitability of glucosensing neurons, increases food intake and body weight
| Autor: | Xi Chen, Rui Wang, Xiaoli Chang, Jun-Hua Yuan, Jing Dong, Liu-Xin Wang, Cai-Shun Zhang, Manwen Li, Kun Zhu, Bin Wang, Qian Lin, Zheng-Yao Jiang |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Food intake food intake Endocrinology Diabetes and Metabolism Growth hormone secretagogue receptor 030209 endocrinology & metabolism lcsh:Diseases of the endocrine glands. Clinical endocrinology 03 medical and health sciences 0302 clinical medicine Endocrinology In vivo Internal medicine energy metabolism Internal Medicine medicine glucose-sensitive neurons Lateral parabrachial nucleus lateral parabrachial nucleus Microinjection Messenger RNA lcsh:RC648-665 business.industry Research digestive oral and skin physiology Electrophysiology 030104 developmental biology ghrelin Ghrelin business |
| Zdroj: | Endocrine Connections Endocrine Connections, Vol 9, Iss 12, Pp 1168-1177 (2020) |
| ISSN: | 2049-3614 |
| Popis: | Ghrelin plays a pivotal role in the regulation of food intake, body weight and energy metabolism. However, these effects of ghrelin in the lateral parabrachial nucleus (LPBN) are unexplored. C57BL/6J mice and GHSR−/− mice were implanted with cannula above the right LPBN and ghrelin was microinjected via the cannula to investigate effect of ghrelin in the LPBN. In vivo electrophysiological technique was used to record LPBN glucose-sensitive neurons to explore potential udnderlying mechanisms. Microinjection of ghrelin in LPBN significantly increased food intake in the first 3 h, while such effect was blocked by [D-Lys3]-GHRP-6 and abolished in GHSR−/− mice. LPBN ghrelin microinjection also significantly increased the firing rate of glucose-excited (GE) neurons and decreased the firing rate of glucose-inhibited (GI) neurons. Additionally, LPBN ghrelin microinjection also significantly increased c-fos expression. Chronic ghrelin administration in the LPBN resulted in significantly increased body weight gain. Meanwhile, no significant changes were observed in both mRNA and protein expression levels of UCP-1 in BAT. These results demonstrated that microinjection of ghrelin in LPBN could increase food intake through the interaction with growth hormone secretagogue receptor (GHSR) in C57BL/6J mice, and its chronic administration could also increase body weight gain. These effects might be associated with altered firing rate in the GE and GI neurons. |
| Databáze: | OpenAIRE |
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