Analysis of globin transition in Xenopus laevis and identification of globins by in vitro translation of hybrid-selected mRNA
| Autor: | D. Gygi, U. Nyffenegger, Rudolf Weber, E. Sandmeier, Toni Wyler |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Messenger RNA
Transition (genetics) media_common.quotation_subject Biophysics Xenopus Translation (biology) Cell Biology Biology (Xenopus laevis)GlobinElectrophoresisHybrid selectionTranslation biology.organism_classification Biochemistry Molecular biology Structural Biology Complementary DNA Genetics Globin Metamorphosis Molecular Biology Polyacrylamide gel electrophoresis media_common |
| Zdroj: | FEBS Letters. (2):219-222 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(86)80901-2 |
| Popis: | The switch from larval to adult globin synthesis during amphibian metamorphosis was analyzed by polyacrylamide gel electrophoresis in acid urea-Triton X-100. 4–6 slowly migrating components were detected in larvae and at least 4 faster components appear at metamorphosis and persist in the adult stage. To relate the globin subunits to the mRNA sequences, isolated as cDNA clones, mRNAs were hybrid-selected from total RNA of larval and adult erythrocytes and translated in a wheat germ system. The translation products were identified by comparison with the globin pattern of hemolysates |
| Databáze: | OpenAIRE |
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