Purification and partial characterization of a mitogenic protein released from preadipocytes of massively obese subjects
| Autor: | Daniel A. K. Roncari, Coral E. Thompson |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
medicine.medical_specialty Stem Cells A protein Cell Differentiation Rats Inbred Strains Cell Biology Cross Reactions Biology Biochemistry Molecular biology Obesity Morbid Rats Endocrinology Adipose Tissue Internal medicine medicine Animals Humans Female Obese subjects Growth Substances Molecular Biology Cell Division Cells Cultured |
| Zdroj: | Biochemistry and Cell Biology. 68:764-768 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/o90-110 |
| Popis: | A protein released into the culture medium by omental preadipocytes of massively obese persons, which stimulates the replication of rat perirenal preadipocytes, has been purified to a high degree. By gel filtration chromatography, the molecular mass of the mitogenic protein was ~66 000 daltons (Da), while on sodium dodecyl sulfate – polyacrylamide gel electrophoresis, two subunits were obtained, relative masses (Mr) of ~31 000 and ~35 000. The isoelectric point of the ~66 000 Da entity was 5.6 ± 0.2. By specific radioreceptor assay, the purified protein was related to epidermal growth factor and transforming growth factor alpha. It was not related to insulin-like growth factors I and II by radioimmunoassay and radioreceptor assay. We propose that the ~66 000 Mr protein, and other mitogenic proteins released by preadipocytes from massively obese persons, act through paracrine–autocrine mechanisms and may play a role in the development of the hyperplasia of enlarged fat cells characteristic of massive corpulence.Key words: preadipocyte, massive obesity, mitogenic, paracrine. |
| Databáze: | OpenAIRE |
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