Single Molecule Imaging of DNA-Protein Interactions Using DNA Curtains

Autor:	J. Brooks Crickard
Rok vydání:	2022
Předmět:	Microscopy Fluorescence Lipid Bilayers Nanotechnology DNA Single Molecule Imaging Article
Zdroj:	Methods Mol Biol Methods in Molecular Biology ISBN: 9781071628461
ISSN:	1940-6029
Popis:	Direct observation of enzymes that work to promote nucleic acid metabolism is a powerful approach to understanding their biochemical and biological properties. Over several years, fluorescent optical microscopy has developed as a powerful tool for watching biological pathways as they occur in real time. Here we describe DNA curtains as an optical microscopy tool that combines engineering, biochemistry, and single molecule imaging to make direct observations of enzymes as they work on DNA in real time. We will provide a detailed methodology of this approach including information about the setup of a basic TIRF microscope, assembly of flow chambers for imaging, and the protocol for making DNA curtains. Our goal is to help the reader better understand the technical approaches to DNA curtains and to better understand the biochemical and biological applications of this approach.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63b397dfd8e155cf94b76cc8a91184f1 https://pubmed.ncbi.nlm.nih.gov/36427147 Zobrazit plný text záznamu