Biomechanical and histological aspects of fracture healing, stimulated with osteogenic protein-1
| Autor: | J. A. M. Bramer, J.M.G.Th Jenner, F. C. den Boer, T. J. Blokhuis, H. J. Th. M. Haarman, Peter Patka, Fred C. Bakker |
|---|---|
| Přispěvatelé: | Other departments |
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Materials science Bone Morphogenetic Protein 7 Biophysics Dentistry Bioengineering Bone healing Bone morphogenetic protein Biomaterials Closed Fracture Fracture Fixation Transforming Growth Factor beta Fracture fixation medicine Animals Fracture Healing business.industry Goats Biomechanics Histology Biomechanical Phenomena Surgery Tibial Fractures Bone morphogenetic protein 7 Mechanics of Materials Bone Morphogenetic Proteins Ceramics and Composites Fracture (geology) Female Collagen business |
| Zdroj: | Biomaterials, 22(7), 725-730. Elsevier BV |
| ISSN: | 0142-9612 |
| Popis: | Fracture healing could be stimulated with osteoinductive bone morphogenetic proteins (bmp's), such as osteogenic protein-1 (OP-1), but little is known about its effectiveness in stimulation of fracture healing. In this study, biomechanical and histological aspects of fracture healing after an injection of OP-1 in the fracture gap were investigated. In 40 goats, a closed fracture was created in the left tibia. The fractures were stabilized with an external fixator and the animals were assigned to four different groups: no injection, injection of 1 mg OP-1, injection of 1 mg OP-1 with collagenous carrier material, and injection of carrier material alone. Twenty-one animals were sacrificed after 2 weeks and 19 after 4 weeks. Biomechanical testing was perfomed on both explanted tibiae. Four longitudinal samples of the fracture were sawn, processed for histology, and examined by two observers. Biomechanical evaluation showed a higher stiffness and strength at 2 weeks after injection of OP-1. Histological evaluation showed normal fracture healing patterns in all animals without adverse effects of the given injections. These data show that fracture healing can be accelerated with a single injection of OP-1, eventually resulting in normally healed bone. |
| Databáze: | OpenAIRE |
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