The Selective Expansion of Functional T Cell Subsets
| Autor: | Danna Lynn Skea, Truman Wong, Robin Hedge, Barbara Dabek, David Nicholson Bell, Brian Wettlaufer |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes T cell Immunology Cell Culture Techniques CD8-Positive T-Lymphocytes Biology Lymphocyte Activation Immunophenotyping Interleukin 21 T-Lymphocyte Subsets medicine Humans Cytotoxic T cell Antigen-presenting cell Cells Cultured Interleukin 3 CD28 Receptors Antigen T-Cell gamma-delta Hematology Natural killer T cell Adoptive Transfer Molecular biology medicine.anatomical_structure Culture Media Conditioned Cytokines Cell Division CD8 |
| Zdroj: | Journal of Hematotherapy & Stem Cell Research. 8:525-538 |
| ISSN: | 1525-8165 |
| DOI: | 10.1089/152581699319975 |
| Popis: | Adoptive cellular immunotherapy is considered a potential treatment for a wide range of diseases, including cancer, infectious diseases, and autoimmune disorders. We have developed a method using a conditioned medium, XLCM, that selectively expands several different T cell subsets with a view to their use in cell therapy. Primary FBS-free suspension cultures of human peripheral blood low-density mononuclear cells treated with XLCM reproducibly expand over 100,000-fold within a period of 4 weeks. CD4+ T cells and CD8+ T cells expand sequentially in the unfractionated cultures, and relatively pure populations of CD4+ and CD8+ T cells may be expanded from populations first enriched in the respective T cell subset. CD4+ T cells cultured in XLCM produce cytokines consistent with the expansion of Th1, Th2, and Th0 subsets, whereas CD8+ T cells cultured in XLCM are cytolytically competent. An interesting feature of T cells cultured in XLCM is the persistence of 5%-10% CD4+CD8+ double-positive T cells in spite of substantial single-positive T cell expansion, suggesting that these cells also proliferate in XLCM. In addition to subsets of TCRalphabeta+ T cells, TCRgammadelta+ T cells are also significantly expanded by XLCM. These results demonstrate that XLCM efficiently expands several functional T cell subsets and provides a means of obtaining selected populations suitable for use in cellular immunotherapy. |
| Databáze: | OpenAIRE |
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