Molecular approaches to the taxonomic position of Peruvian potato cyst nematodes and gene pool similarities in indigenous and imported populations of Globodera
| Autor: | Lionel Renault, Michel Bossis, Eric Grenier, Didier Mugniéry, Didier Fouville |
|---|---|
| Přispěvatelé: | Biologie des organismes et des populations appliquées à la protection des plantes (BIO3P), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2001 |
| Předmět: |
0106 biological sciences
Satellite DNA Molecular Sequence Data Zoology Potato cyst nematode DNA Satellite 01 natural sciences Intraspecific competition Indigenous 03 medical and health sciences Species Specificity RAPD DNA Ribosomal Spacer Peru parasitic diseases Genetics Animals Electrophoresis Gel Two-Dimensional Tylenchoidea Globodera pallida Genes Helminth ComputingMilieux_MISCELLANEOUS Genetics (clinical) Solanum tuberosum 030304 developmental biology 0303 health sciences Base Sequence biology Ecology Genetic Variation Genes rRNA Gene Pool Helminth Proteins DNA Helminth biology.organism_classification DNA Fingerprinting Random Amplified Polymorphic DNA Technique Europe DNA profiling population characteristics Gene pool Polymorphism Restriction Fragment Length [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology geographic locations 010606 plant biology & botany |
| Zdroj: | Heredity Heredity, 2001, 86, pp.277-290 Heredity, Nature Publishing Group, 2001, 86, pp.277-290 |
| ISSN: | 1365-2540 0018-067X |
| Popis: | Peruvian potato cyst nematode populations were analysed to assess both their inter- and intraspecific similarities. ITS--RFLP and two satellite DNA sequences were used as taxonomic tools. Both techniques have confirmed that the Peruvian populations have as their closest relatives the European Globodera pallida, despite the detection of clear differences that prevents us from assigning these South American populations unambiguously to any Globodera species. A more precise study of the variability of these Peruvian populations was investigated and they were compared with the imported European populations using protein (2-DGE) and DNA (RAPD) datasets. The clear distinction between the Peruvian and the European populations was confirmed and, inside each group, no correlation was found between the pathotype classification and the observed clustering of the populations. Surprisingly, while RAPDs revealed a higher variability in the Peruvian group than in the European one, some characteristic proteins were found by 2-DGE in some European populations, whereas it was impossible to find any in the Peruvian populations. It is concluded that the primary founders of the European populations may have an origin other than that of the Peruvian populations involved in this study. |
| Databáze: | OpenAIRE |
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