Studies on the heme and H2-uptake reaction from Azotobacter vinelandii bacterial ferritin
| Autor: | Xiang Chen, Can-He Chen, He-Qing Huang, Zhong Chen, Feng-Zhang Zhang, Qing-Mei Lin |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Iron
Inorganic chemistry Biophysics chemistry.chemical_element Heme Oxygen Electron Transport chemistry.chemical_compound Nitrogen Fixation Electrochemistry Physical and Theoretical Chemistry Electrodes Azotobacter vinelandii biology Nitrogenase Bacterioferritin Hydrogen-Ion Concentration biology.organism_classification Electron transport chain Ferritin Kinetics chemistry Ferritins Nitrogen fixation biology.protein Spectrophotometry Ultraviolet Oxidation-Reduction Hydrogen |
| Zdroj: | Bioelectrochemistry and Bioenergetics. 48:87-93 |
| ISSN: | 0302-4598 |
| DOI: | 10.1016/s0302-4598(98)00215-3 |
| Popis: | Bacterial ferritin of Azotobacter vinelandii (AvBF) is directly able to pick electrons up for iron release from or transfer them for storage to a platinum electrode in the absence of mediator or other reducer. The ferritin containing the structure of heme-Co2+ in part shows weakened activity to electrode and decreases the rate of iron release greatly. A reversible reduction process of the ferritin is observed by the spectral change regularly ranging from 310 to 260 nm under mixed gases containing 98% H2 and 2% to O2. The activity of nitrogen fixation from the whole cell of A. vinelandii increases greatly by H2 reduction with potentials ranging from -397 to -425 mV vs. NHE, indicating two important roles of H2-uptake reaction of the ferritin in increasing activity of nitrogen fixation and in supplying iron to synthesize nitrogenase. |
| Databáze: | OpenAIRE |
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