Bone status of adult female butyrylcholinesterase gene-deficient mice
| Autor: | Katrin S. Lips, Christian Heiss, Lutz Dürselen, Vivien Kauschke, Jonas Sender, Anna Kovtun, Marian Kampschulte, Malte Haupt |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Immunology Real-Time Polymerase Chain Reaction Bone tissue Bone and Bones Gene Expression Regulation Enzymologic Bone remodeling Mice chemistry.chemical_compound Bone Density Osteoclast Internal medicine medicine Animals Immunology and Allergy Butyrylcholinesterase Mice Knockout Pharmacology Cathepsin Bone mineral Osteoblasts Reverse Transcriptase Polymerase Chain Reaction Chemistry Acetylcholinesterase Biomechanical Phenomena medicine.anatomical_structure Endocrinology Female Cortical bone |
| Zdroj: | International Immunopharmacology. 29:208-214 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2015.06.029 |
| Popis: | Butyrylcholinesterase (BChE) degrades acetylcholine in addition to acetylcholinesterase (AChE) which is involved in embryonic development of limbs. Since BChE is expressed by osteoblast-like cells we asked whether it is functional in adult bone remodeling. We addressed this issue by analyzing BChE gene-deficient mice (BChE-KO). Bones were extracted from 16-week old female BChE-KO and corresponding wild type mice (WT). Femoral bones were used for biomechanical testing and μCT evaluation of cancellous and cortical bone. Also vertebrae Th12 and L1 were investigated with μCT while L3 was used for tartrate-resistant acidic phosphatase (TRAP) histomorphometry and Th10 for gene expression analysis by means of real-time RT-PCR. BChE-KO did not reveal significant differences in biomechanical bone strength and bone mineral density determined by μCT. Microarchitecture of cancellous and cortical bone showed an increase in μCT parameters like trabecular thickness, trabecular separation, and relative cortical bone area of femoral BChE-KO bone compared to WT. In vertebrae no changes of microstructure and mRNA expression were detected. However, osteoclast histomorphometry with TRAP stained sections demonstrated a significant increase in relative osteoclast number. In conclusion, in adult murine bone the role of BChE is limited to bone specific changes in microarchitecture and to an increase in relative number of bone resorbing osteoclasts whereas the main collagen resorbing enzyme Cathepsin-K (CtsK) was stably expressed. Besides, AChE might be able to compensate the lack of BChE. Thus, further analyses using bone tissue specific AChE BChE cre–lox double knockout mice would be helpful. |
| Databáze: | OpenAIRE |
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