Clinical use of donation after circulatory death pancreas for islet transplantation
| Autor: | Eelco J.P. de Koning, Marten A. Engelse, Michiel F Nijhoff, Jason B. Doppenberg |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Brain Death endocrine system Tissue and Organ Procurement endocrine system diseases donors and donation Glucose challenge Isolation procedures Urology Islets of Langerhans Transplantation basic (laboratory) research regenerative medicine 030230 surgery 03 medical and health sciences 0302 clinical medicine Diabetes mellitus islet isolation Immunology and Allergy Medicine Humans Pharmacology (medical) Pancreas science Retrospective Studies Transplantation geography geography.geographical_feature_category diabetes business.industry islet transplantation Graft Survival Islet medicine.disease Circulatory death Tissue Donors practice Death medicine.anatomical_structure type 1 clinical research organ acceptance Donation islets of Langerhans business donation after circulatory death (DCD) |
| Zdroj: | American Journal of Transplantation, 21(9), 3077-3087. WILEY American Journal of Transplantation |
| Popis: | Due to a shortage of donation after brain death (DBD) organs, donation after circulatory death (DCD) is increasingly performed. In the field of islet transplantation, there is uncertainty regarding the suitability of DCD pancreas in terms of islet yield and function after islet isolation. The aim of this study was to investigate the potential use of DCD pancreas for islet transplantation. Islet isolation procedures from 126 category 3 DCD and 258 DBD pancreas were performed in a 9-year period. Islet yield after isolation was significantly lower for DCD compared to DBD pancreas (395 515 islet equivalents [IEQ] and 480 017 IEQ, respectively; p = .003). The decrease in IEQ during 2 days of culture was not different between the two groups. Warm ischemia time was not related to DCD islet yield. In vitro insulin secretion after a glucose challenge was similar between DCD and DBD islets. After islet transplantation, DCD islet graft recipients had similar graft function (AUC C-peptide) during mixed meal tolerance tests and Igls score compared to DBD graft recipients. In conclusion, DCD islets can be considered for clinical islet transplantation. |
| Databáze: | OpenAIRE |
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