Photoaffinity labeling of benzophenone-containing salicylanilide compounds to give an insight into the mechanism in disrupting peptidoglycan formation

Autor:	Hao-Chun, Chuang, Ming-Fang, Liu, Hsin-Yi, Wu, Ying-Ta, Wu, Ting-Jen Rachel, Cheng, Jim-Min, Fang
Rok vydání:	2022
Předmět:	Organic Chemistry Clinical Biochemistry Pharmaceutical Science Peptidoglycan Photoaffinity Labels Salicylanilides Biochemistry Molecular Docking Simulation Benzophenones Tandem Mass Spectrometry Drug Discovery Escherichia coli Molecular Medicine Peptidoglycan Glycosyltransferase Molecular Biology
Zdroj:	Bioorganic & Medicinal Chemistry. 67:116819
ISSN:	0968-0896
Popis:	A series of salicylanilide compounds was previously identified as antibacterial agents that inhibit the peptidoglycan formation. To find the exact binding mode, we synthesized a benzophenone-containing salicylanilide compound (1) and used it as a photoaffinity probe to label Acinetobacter baumannii penicillin-binding protein (PBP1b). After incubation and photo-irradiation, the labeled protein was subjected to trypsin digestion, dialysis enrichment, LC-ESI-MS/MS analysis, and Mascot search to reveal an octadecapeptide sequence
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63a416c65c8413675622d5c7046cf770 https://doi.org/10.1016/j.bmc.2022.116819 Zobrazit plný text záznamu Full Text from ScienceDirect