Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum

Autor:	Angel de Jesus Delgado-Bordonave, Itzen Aguiñiga-Sánchez, Ramón Marcos Soto-Hernández, Jorge Cadena-Iñiguez, Victor Manuel Cisneros-Solano, Edelmiro Santiago-Osorio, Víctor Manuel Mendoza-Núñez, Guadalupe Gómez-García, Juana Rosado-Pérez, Edgar Ledesma-Martínez, Mirna Ruiz-Ramos
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	0301 basic medicine Male Pharmaceutical Science Apoptosis High-performance liquid chromatography Mice 0302 clinical medicine Drug Discovery chayote Cytotoxicity Chromatography High Pressure Liquid biology Traditional medicine General Medicine medicine.anatomical_structure 030220 oncology & carcinogenesis Toxicity Molecular Medicine cytotoxicity Female Cucurbitaceae differential Cell Survival Bone Marrow Cells hplc Lethal Dose 50 03 medical and health sciences Inhibitory Concentration 50 Cell Line Tumor Botany medicine Animals gisem Cell Proliferation Pharmacology Dose-Response Relationship Drug Leukemia P388 Plant Extracts Methanol lcsh:RM1-950 biology.organism_classification Antineoplastic Agents Phytogenic In vitro 030104 developmental biology lcsh:Therapeutics. Pharmacology Complementary and alternative medicine Cell culture Fruit Sechium Bone marrow
Zdroj:	Pharmaceutical Biology, Vol 55, Iss 1, Pp 1638-1645 (2017)
ISSN:	1744-5116 1388-0209
Popis:	Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable.
Databáze:	OpenAIRE
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