SAW1 is Required for SDSA Double-Strand Break Repair in S. cerevisiae
| Autor: | Graciel Diamante, Claire Phan, Eric P. Kelson, Angie S. Celis, Paula L. Fischhaber, Jonas Krueger |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Saccharomyces cerevisiae Proteins DNA Repair DNA repair Saccharomyces cerevisiae RAD52 Biophysics Biochemistry DNA-binding protein Article Endonuclease chemistry.chemical_compound DNA Breaks Double-Stranded Molecular Biology biology fungi Single-Strand Specific DNA and RNA Endonucleases Cell Biology Repair site biology.organism_classification Molecular biology Double Strand Break Repair Cell biology DNA-Binding Proteins chemistry biology.protein DNA Gene Deletion |
| Popis: | SAW1, coding for Saw1, is required for single-strand annealing (SSA) DNA double-strand break (DSB) repair in Saccharomycescerevisiae. Saw1 physically associates with Rad1 and Rad52 and recruits the Rad1-Rad10 endonuclease. Herein we show by fluorescence microscopy that SAW1 is similarly required for recruitment of Rad10 to sites of Synthesis-Dependent Strand Annealing (SDSA) and associates with sites of SDSA repair in a manner temporally overlapped with Rad10. The magnitude of induction of colocalized Saw1-CFP/Rad10-YFP/DSB-RFP foci in SDSA is more dramatic in S and G2 phase cells than in M phase, consistent with the known mechanism of SDSA. We observed a substantial fraction of foci in which Rad10 was localized to the repair site without Saw1, but few DSB sites that contained Saw1 without Rad10. Together these data are consistent with a model in which Saw1 recruits Rad1-Rad10 to SDSA sites, possibly even binding as a protein-protein complex, but departs the repair site in advance of Rad1-Rad10. |
| Databáze: | OpenAIRE |
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