Haematological and CD4+ T cells reference ranges in healthy adult populations in Gojjam zones in Amhara region, Ethiopia

Autor: Mulat Yimer, Zewdie Mekonnen, Yesuf Adem, Wondimu Gebeyehu, Wondemagegn Mulu, Yohannes Zenebe, Asmare Amuamuta, Bayeh Abera
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
Veterinary medicine
Percentile
Research Facilities
Cross-sectional study
Physiology
Neutrophils
lcsh:Medicine
Hematocrit
Geographical Locations
White Blood Cells
Hemoglobins
0302 clinical medicine
Animal Cells
Reference Values
Medicine and Health Sciences
Medicine
Ethnicities
030212 general & internal medicine
Young adult
lcsh:Science
education.field_of_study
Multidisciplinary
medicine.diagnostic_test
T Cells
Hematology
Healthy Volunteers
Body Fluids
medicine.anatomical_structure
Blood
Female
Cellular Types
Anatomy
Research Laboratories
Research Article
Platelets
Adult
T cell
Immune Cells
030231 tropical medicine
Population
Immunology
Research and Analysis Methods
03 medical and health sciences
Young Adult
Humans
education
Blood Cells
business.industry
lcsh:R
Becton dickinson
Biology and Life Sciences
Cell Biology
CD4 Lymphocyte Count
Blood Counts
Cross-Sectional Studies
People and Places
Africa
Erythrocyte Count
lcsh:Q
Population Groupings
Hemoglobin
Ethiopia
business
Africans
Government Laboratories
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 7, p e0181268 (2017)
ISSN: 1932-6203
Popis: Introduction Establishing national population haematological and immunological reference ranges are essential for clinical management of patients. However, there is scarcity of information on community based haematological reference ranges established from Ethiopian population. Therefore, this study aimed at determining haematological and CD4+ T cells reference ranges in healthy adults from East and West Gojjam zones, Ethiopia. Methods Community based cross-sectional study was conducted from May 2015 to December 2015 in healthy adult residents of Gojjam zone. A total of 481(246 females and 235 males) healthy adults enrolled in the study. Healthy adults were defined by medical history, physical examination and laboratory screening for HIV, HBV, HCV and intestinal parasitosis. Haematological parameters were measured using haematology analyzer MindrayBC320 (Mindray Biomedical electronic Corporation, China). CD4+Tcells were enumerated using FACS count (Becton Dickinson). Results The median age of the participants was 25 years. The overall median and 95th percentile of CD4+ T cells count were 869 cells/mm3 and396–1598 cells/mm3, respectively. Females had a significantly higher CD4+ T cell counts compared to males (P = 0.002). The 95th percentile range for red blood cells (RBCs) was 3.93–6.1 x 106cells/mm3and for hematocrit (Hct) was 40–58% while for hemoglobin (Hb) was 15.69–17.84g/dl. Males had significantly higher values of RBC and Hct than females (P < 0.001). Females (120–379 x 106 cells/mm3) had significantly higher platelet counts than males (106–352 x106 cells/mm3) (P < 0.001). The overall median of WBC was6.78 x103/mm3and its95thpercentile range was3.5–11.5 x103/mm3. The overall 95th percentile range of MCV, MCH and MCHC were 89.5–107.5 fl, 28–34 pg and 30–33.2g/dl, respectively. The higher mean absolute count of RBCs was found in the youngest age groups (P = 0.03). The mean count of RBCs and Hct were significantly higher in highschool completed and above than other participants (P < 0.001). The lower and upper limit of platelet counts was significantly higher in highland (118 -383x106 cells/mm3) compared to lowland residents (107–352 x106 cells/mm3) (P < 0.001). Moreover, it was significantly higher in residents with better monthly income (124–383 x106 cells/mm3) compared to the counters (115–368 x106 cells/mm3) (P = 0.02). Conclusions Some of the haematological and CD4+ T cells reference ranges of the healthy adults in this study showed variations with the reference ranges used and reported so far in Ethiopia, Africa and Western countries. We recommend further study considering gender, altitude, and residency in other parts of Ethiopia to establish national reference ranges for Ethiopian population.
Databáze: OpenAIRE
Externí odkaz: