Microbial Diversity Similarities in Periodontal Pockets and Atheromatous Plaques of Cardiovascular Disease Patients

Autor: Reginaldo Bruno Gonçalves, Antonio Wilson Sallum, Humberto M. Passos, Renato Corrêa Viana Casarin, Eduardo L. Nicolela Junior, Wagner Leal Serra e Silva Filho
Rok vydání: 2014
Předmět:
Male
Pathology
Gingival and periodontal pocket
Molecular biology
lcsh:Medicine
DNA cloning
Coronary Artery Disease
Coronary artery disease
Sequencing techniques
Oral Diseases
Medicine and Health Sciences
Coronary Heart Disease
DNA sequencing
Cloning
Molecular
lcsh:Science
Phylogeny
Multidisciplinary
biology
Biodiversity
Middle Aged
Plaque
Atherosclerotic
medicine.anatomical_structure
Cardiovascular Diseases
Plasmid Sequencing
Female
Research Article
DNA
Bacterial
medicine.medical_specialty
Aggregatibacter
Oral Medicine
Cardiology
Veillonella
Research and Analysis Methods
Bacterial Physiological Phenomena
Microbiology
medicine
Humans
Periodontal Pocket
Moraxella
Periodontal Diseases
Periodontitis
Biology and life sciences
Bacteria
lcsh:R
Sequence Analysis
DNA
medicine.disease
biology.organism_classification
Coronary arteries
Molecular biology techniques
Atheroma
Biofilms
lcsh:Q
Cloning
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 10, p e109761 (2014)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0109761
Popis: Background and Objective The immune and infectious alterations occurring in periodontitis have been shown to alter the development and severity of cardiovascular disease. One of these relationships is the translocation of oral bacteria to atheroma plaques, thereby promoting plaque development. Thus, the aim of this study was to assess, by 16s cloning and sequencing, the microbial diversity of the subgingival environment and atheroma plaques of patients concomitantly suffering from periodontitis and obstructive coronary artery atherosclerosis (OCAA). Methods Subgingival biofilm and coronary balloons used in percutaneous transluminal coronary angioplasty were collected from 18 subjects presenting with generalized moderate to severe periodontitis and OCAA. DNA was extracted and the gene 16S was amplified, cloned and sequenced. Results Significant differences in microbial diversity were observed between both environments. While subgingival samples mostly contained the phylum Firmicutes, in coronary balloons, Proteobacteria (p
Databáze: OpenAIRE
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