Localized sinus inflammation in a rabbit sinusitis model induced by Bacteroides fragilis is accompanied by rigorous immune responses
| Autor: | Sining Sun, Keith C. Kajander, Harumi Jyonouchi, Frank L. Rimell, G R Germaine, James R. Miller, Cynthia A. Kennedy, Anie K. Roche |
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| Rok vydání: | 1999 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty 040301 veterinary sciences Blotting Western Inflammation Enzyme-Linked Immunosorbent Assay 0403 veterinary science Bacteroides fragilis 03 medical and health sciences Immune system Medicine Animals Sinusitis Sinus (anatomy) Immunity Cellular biology business.industry food and beverages Histology 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Bacteroides Infections Antibodies Bacterial 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology Apoptosis Immunoglobulin G Immunology Antibody Formation Chronic Disease Surgery Rabbits medicine.symptom Bacteroides business |
| Zdroj: | Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery. 120(6) |
| ISSN: | 0194-5998 |
| Popis: | We evaluated inflammatory and immune responses against Bacteroides fragilis in a rabbit sinusitis model. Bacteroides was inoculated into the left maxillary sinus, and inflammatory (histology, cell number/cytology, lactose dehydrogenase, and apoptosis) and immune responses in the sinus, airway, and peripheral blood (PB) were determined for up to 4 weeks. In the inflamed sinus, the lactose dehydrogenase level was markedly elevated, with neutrophilic infiltration, severe tissue inflammation, and increased apoptosis. Low-grade tissue inflammation was present in the contralateral and sham-operated sinuses, but other parameters remained unchanged, and so did those in the airway and PB in the inoculated rabbits. Serum IgG antibody levels increased rapidly, were highest at 3 weeks, and began to decline at 4 weeks. Cellular immune responses (proliferation and interferon-γ mRNA expression) against Bacteroides were detected in the PB of all inoculated rabbits. Vigorous immune responses against Bacteroides may have localized but failed to terminate inflammation in the sinus, indicating importance of microenvironmental factors. (Otolaryngol Head Neck Surg 1999;120:869-75.) |
| Databáze: | OpenAIRE |
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