Analytical evaluation of a rapid on-site oral fluid drug test
Autor: | Alain Verstraete, An-Sofie Goessaert, Jolien Veramme, Kristof Pil |
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Rok vydání: | 2010 |
Předmět: |
Automobile Driving
Validation study Saliva DEVICES Street drugs Drug Evaluation Preclinical Poison control Pharmacology Sensitivity and Specificity Biochemistry Analytical Chemistry Sensitivity DRIVERS Medicine and Health Sciences medicine Drug test Amphetamine Point-of-care test Varian Oralab (R) 6 Chromatography medicine.diagnostic_test Illicit Drugs business.industry Reproducibility of Results Equipment Design Forensic Medicine Methamphetamine Equipment Failure Analysis Europe Oral fluid business medicine.drug |
Zdroj: | ANALYTICAL AND BIOANALYTICAL CHEMISTRY |
ISSN: | 1618-2650 1618-2642 |
Popis: | There is a need for a reliable rapid on-site oral fluid test that can be used in police controls to detect impaired drivers. We evaluated the Varian OralabA (R) 6 and collected two oral fluid samples from 250 subjects, one with the Varian OralabA (R) 6 and one with the StatSure (TM) SalivaaEuro cent Sampler (TM). The OralabA (R) 6 can detect six drug types: amphetamines, methamphetamine, cocaine, opiates, delta9-tetrahydrocannabinol (THC), and phencyclidine (PCP). On-site results were obtained within 10 to 15 min. The sample collected with StatSure (TM) was analyzed using liquid chromatography-tandem mass spectrometry after liquid-liquid extraction and these results were used as a reference to determine prevalence, sensitivity, and specificity. Two cut-off values were used in the evaluation. The Varian cut-off values were: amphetamine 50 ng/mL, cocaine 20 ng/mL, opiates 40 ng/mL, and THC 50 ng/mL. The DRUID cut-offs were: amphetamine 25 ng/mL, cocaine 20 ng/mL, opiates 20 ng/mL, and THC 1 ng/mL. Applying the first cut-offs, prevalence, sensitivity, and specificity were: amphetamine 10%, 76%, 100%; cocaine 23%, 34%, 100%; opiates 38%, 83%, 94%; and THC 18%, 41%, 99%. The DRUID cut-off values gave the following results: amphetamine 14%, 56%, 100%; cocaine 28%, 34%, 100%; opiates 49%, 68%, 98%, and THC 45%, 16%, 99%. The specificity of the OralabA (R) 6 is generally good. For both cut-offs, sensitivity was low for cocaine and THC. Therefore, the Varian OralabA (R) 6 test is not sensitive enough to be applied during roadside police controls. |
Databáze: | OpenAIRE |
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