Factors controlling the DNA-synthesis in 3T3 and EAT cells
| Autor: | H. Thomou, D. Stathakos, S. Koussoulakos |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Hot Temperature
Globulin Physiology Beta-Globulins Beta globulins Biochemistry chemistry.chemical_compound Mice Biosynthesis Animals Carcinoma Ehrlich Tumor Growth Substances Molecular Biology Cells Cultured biology DNA synthesis Isoelectric focusing Cationic polymerization General Medicine DNA DNA Neoplasm Embryo Mammalian Growth Inhibitors chemistry Cell culture biology.protein Isoelectric Focusing |
| Zdroj: | Comparative biochemistry and physiology. B, Comparative biochemistry. 80(1) |
| ISSN: | 0305-0491 |
| Popis: | 1. 1. Beta globulins, (Cohn Fr. III), are a major source of molecules affecting the DNA-synthesis of 3T3 and EAT cells. 2. 2. Growth inhibitors for both cell types, chromatograph at the same position, corresponding to a mol. wt of about 50,000. 3. 3. A very basic, (pI 10.1), factor is isolated by gel electrofocusing, which stimulates the DNA-synthesis of 3T3 and EAT cells. Because of its extremely high cationic charge and its adsorption on gels, the estimation of the exact molecular weight and its preparative isolation, becomes very difficult. 4. 4. Some of the above mentioned molecules are heat-stable and express their action even after boiling for 10 min at pH 3. |
| Databáze: | OpenAIRE |
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