LncRNA AGAP2-AS1 augments cell viability and mobility, and confers gemcitabine resistance by inhibiting miR-497 in colorectal cancer
| Autor: | Shiquan Li, Zhenkun Yan, Sen Hong, Miaomiao Bi, Yumei Song |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Antimetabolites Antineoplastic Aging Carcinogenesis Cell Survival Colorectal cancer Apoptosis Biology miR-497 medicine.disease_cause Deoxycytidine colorectal cancer (CRC) survival Metastasis Cell Movement Cell Line Tumor medicine Humans Viability assay Cell Proliferation Cell growth Fibroblast growth factor receptor 1 Oncogenes Cell Biology Middle Aged Prognosis medicine.disease Gemcitabine digestive system diseases Up-Regulation Antisense RNA Gene Expression Regulation Neoplastic long non-coding RNAs (lncRNAs) MicroRNAs Drug Resistance Neoplasm Cancer research Female RNA Long Noncoding fibroblast growth factor receptor 1 (FGFR1) Colorectal Neoplasms Research Paper medicine.drug |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| DOI: | 10.18632/aging.102940 |
| Popis: | Background Most recently, long non-coding RNAs (lncRNAs) emerge as crucial modulators in many biological processes, such as embryonic development, cell growth, and tumorigenesis. However, the correlations between lncRNAs and colorectal cancer (CRC) cell proliferation, metastasis, and gemcitabine resistance are not well understood. Results The expression of AGAP2-AS1 was overexpressed in CRC tissues and negatively correlated with the survival of patients with CRC. AGAP2-AS1 promoted CRC cell proliferation and inhibited apoptosis. Moreover, AGAP2-AS1 enhanced the chemoresistance of CRC cells to gemcitabine. In addition, AGAP2-AS1 enhanced the migration and invasion of CRC cells. Mechanistic studies showed that AGAP2-AS1 regulated fibroblast growth factor receptor 1 (FGFR1) expression by sponging miR-497 in CRC progression. Conclusion We identified an oncogenic role of AGAP2-AS1 in the development and progression of CRC. Methods qRT-PCR was used to measure the expression of AGAP2 Antisense RNA 1 (AGAP2-AS1) in 116 cases of CRC and adjacent normal tissues. Luciferase reporter assays was used to detect the interaction between AGAP2-AS1 and miR-497. The xenograft tumor experiment was used to study the in vivo function of AGAP2-AS1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|