Association between polymorphisms of eNOS and GPx-1 genes, activity of free-radical processes and in-stent restenosis
| Autor: | R. O. Shirokov, A. N. Samko, Alexey N Meshkov, A. I. Kaminnyi, Yu. A. Shuvalova |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Lipid Peroxides medicine.medical_specialty Erythrocytes Free Radicals Nitric Oxide Synthase Type III Clinical Biochemistry Coronary Angiography Polymorphism Single Nucleotide Coronary Restenosis Superoxide dismutase Glutathione Peroxidase GPX1 Restenosis Enos Internal medicine Genotype medicine Humans Genetic Predisposition to Disease Allele Molecular Biology Genetic Association Studies chemistry.chemical_classification Genetics Glutathione Peroxidase biology Glutathione peroxidase Cell Biology General Medicine Middle Aged biology.organism_classification medicine.disease Minor allele frequency Endocrinology chemistry biology.protein Stents Lipoprotein |
| Zdroj: | Molecular and Cellular Biochemistry. 370:241-249 |
| ISSN: | 1573-4919 0300-8177 |
| Popis: | Our aim was to examine correlations between polymorphisms in five antioxidant enzymes genes, activity of free-radical processes, and the risk of restenosis after coronary artery stenting with bare metal stents (BMS). A total of 101 male patients who underwent intracoronary stenting using BMS and coronary angiography follow-up of 6 months were enrolled in: group with in-stent restenosis (n = 44) and without restenosis (n = 57). The content of lipoperoxides and malondialdehyde (MDA) in Low-density lipoprotein (LDL), activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) in erythrocytes, and genotypes polymorphisms of the CAT gene (-262C/T), paraoxonase-1 (PON-1) gene (163T/A and 575A/G), endothelial nitric oxide synthase (eNOS) gene (298G/T (rs#1799983) and -786T/C), GPx-1 gene (599C/T (rs#1050450)), and glutathione-S-transferase (GSTP) gene (313A/G) were determined. In carriers of the minor allele of 599C/T polymorphism of the GPx-1 gene, activity of GPx in erythrocytes was lower by 17 % than in wild allele homozygotes, while the content of lipoperoxides in LDL was higher by 74 %. T-allele of 599C/T polymorphism of the GPx-1 gene (OR = 2.9; 95 % CI: 1.23-6.82) and T-allele of 298G/T polymorphism of the eNOS gene (OR = 2.79; 95 % CI: 1.17-6.66) were associated with the risk of in-stent restenosis. Minor alleles of polymorphisms 298G/T of the eNOS gene and 599C/T of the GPx-1 gene are associated with an increased risk of in-stent restenosis. Minor allele of the GPx-1 gene 599C/T polymorphism leads to a decrease of the GPx activity and increase of the activity of free-radical processes. |
| Databáze: | OpenAIRE |
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