Modified and Mutant Porins in the Study on Molecular Basis of Non- Specific Diffusion
| Autor: | Tamara F. Solov'eva, Olga Portnyagina, Olga D. Novikova |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cell Membrane Permeability
Membrane permeability Chemistry Mutant Conductance Mutagenesis (molecular biology technique) Porins Cell Biology General Medicine Gating Biochemistry Transmembrane protein Electrophysiological Phenomena Diffusion Crystallography Porin Mutation Biophysics Mutagenesis Site-Directed bacteria Site-directed mutagenesis Molecular Biology Bacterial Outer Membrane Proteins |
| Zdroj: | Current proteinpeptide science. 18(3) |
| ISSN: | 1875-5550 |
| Popis: | Site-directed mutagenesis allows elucidation of the basic principles of the porin-driven membrane permeability and opens the possibility for the modulation of functional states of porin channels. The review is aimed to show the advantages of using mutant and chemically modified porins for obtaining detailed information about molecular mechanisms that underlie the non-specific transmembrane diffusion. We summarized data regarding the effects of the point substitutions and the external loop deletions on electrophysiological properties of general porins. The influence of charges inside the pore eyelet and the roles of external loops in ion conductance, ion selectivity, and voltage gating were described. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|