Production of polyclonal and monoclonal antibodies against group A, B, and C capsular polysaccharides of Neisseria meningitidis and preparation of latex reagents
| Autor: | D. Postic, Martine Guibourdenche, Jean-Yves Riou, J. C. Mazie, Jean-Michel Fournier, N Sagot, Faridabano Nato, B Slizewicz |
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| Rok vydání: | 1991 |
| Předmět: |
Microbiology (medical)
medicine.drug_class Meningitis Meningococcal Neisseria meningitidis medicine.disease_cause Monoclonal antibody Microbiology Antigen medicine Humans Serotyping Antigens Bacterial biology Polysaccharides Bacterial Antibodies Monoclonal biology.organism_classification Antibodies Bacterial Latex fixation test Polyclonal antibodies Evaluation Studies as Topic Monoclonal biology.protein Neisseriaceae Indicators and Reagents Antibody Latex Fixation Tests Research Article |
| Zdroj: | Journal of clinical microbiology. 29(7) |
| ISSN: | 0095-1137 |
| Popis: | Polyclonal and monoclonal antibodies against capsular polysaccharides of Neisseria meningitidis serogroups A, B, and C were produced in order to develop immunological reagents allowing both the detection of soluble antigens during meningococcal meningitis and antigenic serogrouping of N. meningitidis cultures. The performance characteristics of monoclonal and polyclonal antibody latex reagents were compared. For the detection of soluble polysaccharide antigen, polyclonal antibody latex reagent was selected for N. meningitidis A and C. The latex reagent prepared with polyclonal antibodies against N. meningitidis B could not detect capsular polysaccharide even at 1 mg/ml. The monoclonal antibody B latex reagent which detected 100 ng of polysaccharide per ml was therefore chosen. For the serogroup identification of N. meningitidis, the use of a confirmatory test results in an overall specificity of 100% with polyclonal or monoclonal antibody latex reagents. |
| Databáze: | OpenAIRE |
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