A Nucleoprotein-Enriched Diet Suppresses Dopaminergic Neuronal Cell Loss and Motor Deficit in Mice with MPTP-Induced Parkinson’s Disease
Autor: | Natsuki Kobayashi, Chika Sawa, Shun Sasaki, Seiji Shioda, Keisuke Kiriyama, Norimitsu Murai, Masaji Matsunaga, Hirokazu Ohtaki, Kazue Satoh, Minako Matsumoto |
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Rok vydání: | 2014 |
Předmět: |
Male
medicine.medical_specialty Parkinson's disease Tyrosine 3-Monooxygenase Substantia nigra Biology medicine.disease_cause Electron Transport Complex IV Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Mesencephalon Superoxides Internal medicine medicine Animals Tyrosine hydroxylase Pars compacta Superoxide Dopaminergic Neurons MPTP Dopaminergic MPTP Poisoning General Medicine medicine.disease Mice Inbred C57BL Nucleoproteins Endocrinology chemistry Dietary Supplements Locomotion Oxidative stress |
Zdroj: | Journal of Molecular Neuroscience. 55:803-811 |
ISSN: | 1559-1166 0895-8696 |
DOI: | 10.1007/s12031-014-0432-2 |
Popis: | Parkinson's disease (PD) is an obstinate progressive neurodegenerative disease and characterized by locomotor impairment and dopaminergic neuronal degeneration in the substantia nigra pars compacta (SNc). We examined in here the dietary effect of nucleoprotein (NP) extracted from salmon soft roe on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-injected PD-like mice model to prevent the symptom as an alternative medicine. Male C57/BL6 mice were given either an artificially modified NP-free diet (NF) or NF supplied with 1.2% NP for 1 week. Then, mice were injected intraperitoneally four times with 20 mg/kg MPTP. Seven days later, locomotor activity was examined, and the brains were immunostained with tyrosine hydroxylase (TH) and Iba1 antibodies. Moreover, in situ detection of superoxide anion (O2(-)) and gene expression of mitochondrial electron transfer chain gene, Cox8b was evaluated in midbrains. NP-fed animals showed significantly reduced locomotor impairment and an increased number of TH-positive cells in the SNc compared with NF animals. The NP-fed animals also showed reduced lower levels of O2(-) and up-regulation of Cox8b levels and Iba1 immunoreactivity, suggesting that inflammation and oxidative stress were suppressed and mitochondrial impairment was relieved in these animals. Supplementation of the diet with NP may serve as a useful preventive measure to slow the onset of PD. |
Databáze: | OpenAIRE |
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