A novel antioxidant ergothioneine PET radioligand for in vivo imaging applications
| Autor: | Mohammed N. Tantawy, Todd E. Peterson, Clayton A. Whitmore, Robert B. Beelman, Justin R. Haynes, Fiona E. Harrison, Adam J. Rosenberg, Wellington Pham, William J. Behof |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biodistribution
Science Pharmacology Article Antioxidants chemistry.chemical_compound Mice Pharmacokinetics In vivo Radioligand Animals Tissue Distribution Carbon Radioisotopes chemistry.chemical_classification Multidisciplinary Ergothioneine In vitro Amino acid Mice Inbred C57BL Chemistry chemistry Positron-Emission Tomography Medicine Radiopharmaceuticals Preclinical imaging Biomarkers Biotechnology Neuroscience |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Ergothioneine (ERGO) is a rare amino acid mostly found in fungi, including mushrooms, with recognized antioxidant activity to protect tissues from damage by reactive oxygen species (ROS) components. Prior to this publication, the biodistribution of ERGO has been performed solely in vitro using extracted tissues. The aim of this study was to develop a feasible chemistry for the synthesis of an ERGO PET radioligand, [11C]ERGO, to facilitate in vivo study. The radioligand probe was synthesized with identical structure to ERGO by employing an orthogonal protection/deprotection approach. [11C]methylation of the precursor was performed via [11C]CH3OTf to provide [11C]ERGO radioligand. The [11C]ERGO was isolated by RP-HPLC with a molar activity of 690 TBq/mmol. To demonstrate the biodistribution of the radioligand, we administered approximately 37 MBq/0.1 mL in 5XFAD mice, a mouse model of Alzheimer’s disease via the tail vein. The distribution of ERGO in the brain was monitored using 90-min dynamic PET scans. The delivery and specific retention of [11C]ERGO in an LPS-mediated neuroinflammation mouse model was also demonstrated. For the pharmacokinetic study, the concentration of the compound in the serum started to decrease 10 min after injection while starting to distribute in other peripheral tissues. In particular, a significant amount of the compound was found in the eyes and small intestine. The radioligand was also distributed in several regions of the brain of 5XFAD mice, and the signal remained strong 30 min post-injection. This is the first time the biodistribution of this antioxidant and rare amino acid has been demonstrated in a preclinical mouse model in a highly sensitive and non-invasive manner. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink