Outbreak of Salmonella enterica serotype Infantis producing ArmA 16S RNA methylase and CTX-M-15 extended-spectrum beta-lactamase in a neonatology ward in Constantine, Algeria
| Autor: | Abdesselam Lezzar, Gaelle Cuzon, Farida Smati, Chafia Bentchouala, Thierry Naas, Sanàa Yaou, Patrice Nordmann |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Serotype Salmonella Genotype medicine.disease_cause beta-Lactamases Microbiology Disease Outbreaks 03 medical and health sciences Feces Plasmid Drug Resistance Multiple Bacterial medicine Cluster Analysis Humans Pharmacology (medical) Typing Child 030304 developmental biology 0303 health sciences tRNA Methyltransferases Gastric Juice biology 030306 microbiology Outbreak Infant Salmonella enterica General Medicine Ribosomal RNA biology.organism_classification Enterobacteriaceae Virology 3. Good health Anti-Bacterial Agents Hospitalization Molecular Typing Infectious Diseases Blood Algeria Child Preschool Salmonella Infections Plasmids |
| Zdroj: | International Journal of Antimicrobial Agents; Vol 38 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2011.04.012 |
| Popis: | Plasmid-mediated 16S rRNA methylases such as ArmA, which confer high levels of resistance to aminoglycosides, are increasingly reported in Enterobacteriaceae. This study investigated the molecular mechanism of β-lactam and aminoglycoside resistance in extended-spectrum β-lactamase (ESBL)-producing Salmonella enterica serotype Infantis isolated at the 53-bed neonatology ward of University Hospital Benabib in Constantine, Algeria. From September 2008 to January 2009, 200 S. enterica isolates were obtained from 138 patients (age range 8–80 months) hospitalised in the neonatology ward. Most isolates were from stool cultures, but also from two blood cultures and one gastric fluid. The isolates were multidrug-resistant and produced TEM-1 and CTX-M-15 enzymes as well as the 16S RNA methylase ArmA. The armA , bla CTX-M-15 and bla TEM-1 genes were located on the same 140-kb self-transferable plasmid belonging to the IncL/M incompatibility group. All of the S. Infantis isolates belonged to a single clone. Increased infection control measures and thorough biodecontamination of the rooms led to control of the outbreak but did not eradicate the epidemic strain. This study further illustrates the global emergence of ArmA methylase and its frequent association with bla CTX-M genes. Spread of 16S RNA methylase determinants at the same level as bla CTX-M genes in Enterobacteriaceae may seriously compromise the efficacy of aminoglycosides for treating Gram-negative infections. |
| Databáze: | OpenAIRE |
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