Ultrastructure changes associated with brain death in the human donor heart
| Autor: | Y. Ye, K. W. Min, Dimitri Novitzky, J. Rhodin, David K. C. Cooper, L. E. Debault |
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| Rok vydání: | 1997 |
| Předmět: |
Nephrology
Inotrope Adult Male medicine.medical_specialty Pathology Brain Death Adolescent Sarcomere Central nervous system disease Internal medicine Medicine Humans Child Transplantation business.industry Myocardium medicine.disease Tissue Donors Microscopy Electron medicine.anatomical_structure Ventricle Circulatory system Ultrastructure Heart Transplantation Female business |
| Zdroj: | Transplant international : official journal of the European Society for Organ Transplantation. 10(1) |
| ISSN: | 0934-0874 |
| Popis: | Electromicroscopic examinations were carried out on 30 myocardial biopsies taken from 22 human donor hearts immediately after excision (prestorage) or immediately before transplantation (post-storage). All electron micrographs were independently examined by two morphologists. Eleven structures were examined in each micrograph, and each structure was scored according to the degree of injury. A good interobserver correlation was obtained in 84 % of the structures scored. In the prestorage left ventricular biopsies (n = 11), approximately 20 %–25 % showed moderate to severe ultrastructural injury. The ultrastructural injury observed in the poststorage left ventricular biopsies (n = 15) was no different from that in the prestorage group, particularly injury to the sarcomere and mitochondria. A similar degree and pattern of injury was seen in the right ventricle (n = 4). There was no evidence that an ischemic storage period of less than 6 h increased the degree of injury seen. However, there was a higher incidence of moderate to severe injury in those hearts excised from donors initially dependent on high inotropic support. |
| Databáze: | OpenAIRE |
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