Fibroblast growth factor receptor 2 expression in apical periodontitis in mice
| Autor: | Stephanie Díaz Huamán, Sandra Y. Fukada, Paulo Nelson-Filho, R. A. da Silva, A. De Rossi, Jorge Esquiche León, Maria da Conceição Pereira Saraiva, F. de Carvalho |
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| Rok vydání: | 2020 |
| Předmět: |
musculoskeletal diseases
Pathology medicine.medical_specialty 0206 medical engineering Osteoclasts 02 engineering and technology Bone resorption Mice 03 medical and health sciences 0302 clinical medicine stomatognathic system Osteoclast Cathepsin K Animals Medicine Cementum Receptor Fibroblast Growth Factor Type 2 General Dentistry Dental alveolus Dental Cementum Periodontitis biology business.industry OSTEOCLASTO 030206 dentistry medicine.disease 020601 biomedical engineering Root Canal Therapy stomatognathic diseases medicine.anatomical_structure RANKL biology.protein Pulp (tooth) business Periapical Periodontitis |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1365-2591 0143-2885 |
| DOI: | 10.1111/iej.13315 |
| Popis: | AIM To investigate the presence, localization and the possible correlation of the fibroblast growth factor receptor-2 (FGFR2) with inflammatory resorption of cementum, periodontal ligament and alveolar bone during development of apical periodontitis in mice. METHODOLOGY Apical periodontitis was experimentally induced in mandibular first molars of mice by pulp exposure to the oral environment. Healthy teeth without pulp exposure were used as controls. At 7, 21 and 42 days following pulp exposure, the animals were euthanized and the jaws were prepared for analysis under conventional and fluorescence microscopy, immunohistochemistry (FGFR2), RT-PCR (RNAm levels of RANK, RANKL, OPG, Runx2 and cathepsin K) and enzyme histochemistry (cementoclasts and osteoclasts). Statistical analysis was performed by Kruskal-Wallis tests and Dunn's post hoc tests for multiple comparisons (α = 0.05) using SAS 9.4 software. RESULTS FGFR2-positive cells were not observed in the tissues surrounding healthy teeth but were observed in teeth with periapical lesions from seven days after root canal contamination. At days 21 and 42 after endodontic infection, the increase in periapical lesion size was accompanied by significantly enhanced expression of FGFR2 (P |
| Databáze: | OpenAIRE |
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