Evaluation of SSYA10-001 as a Replication Inhibitor of Severe Acute Respiratory Syndrome, Mouse Hepatitis, and Middle East Respiratory Syndrome Coronaviruses

Autor: Kamalendra Singh, Susan R. Weiss, Matthew B. Frieman, Adeyemi O. Adedeji, Ruth Elliott, Stefan G. Sarafianos, Ademola Kassim, Christopher M. Coleman
Rok vydání: 2014
Předmět:
Middle East respiratory syndrome coronavirus
viruses
Molecular Sequence Data
Biology
Virus Replication
medicine.disease_cause
Antiviral Agents
Cell Line
Inhibitory Concentration 50
Mice
Viral Proteins
Mouse hepatitis virus
Chlorocebus aethiops
medicine
Animals
Humans
Pharmacology (medical)
Amino Acid Sequence
Respiratory system
Vero Cells
Coronavirus
Pharmacology
Murine hepatitis virus
Binding Sites
Dose-Response Relationship
Drug
Sequence Homology
Amino Acid
DNA Helicases
virus diseases
Helicase
respiratory system
Fibroblasts
Triazoles
biochemical phenomena
metabolism
and nutrition
medicine.disease
biology.organism_classification
Virology
respiratory tract diseases
Molecular Docking Simulation
Infectious Diseases
Severe acute respiratory syndrome-related coronavirus
Viral replication
Middle East Respiratory Syndrome Coronavirus
biology.protein
Vero cell
Middle East respiratory syndrome
Sequence Alignment
Protein Binding
Zdroj: Antimicrobial Agents and Chemotherapy
ISSN: 1098-6596
0066-4804
Popis: We have previously shown that SSYA10-001 blocks severe acute respiratory syndrome coronavirus (SARS-CoV) replication by inhibiting SARS-CoV helicase (nsp13). Here, we show that SSYA10-001 also inhibits replication of two other coronaviruses, mouse hepatitis virus (MHV) and Middle Eastern respiratory syndrome coronavirus (MERS-CoV). A putative binding pocket for SSYA10-001 was identified and shown to be similar in SARS-CoV, MERS-CoV, and MHV helicases. These studies show that it is possible to target multiple coronaviruses through broad-spectrum inhibitors.
Databáze: OpenAIRE
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