Genetic diversity of drug and multidrug-resistant Mycobacterium tuberculosis circulating in Veracruz, Mexico

Autor: José Zarrabal-Meza, Javier Fuentes-Domínguez, Ma. Teresa Martínez-Cazares, Marie Nancy Séraphin, Aurora Parissi-Crivelli, Roberto Zenteno-Cuevas, Daniela Munro-Rojas, Jorge A. Gonzalez-y-Merchand, Esdras Fernandez-Morales, Michael Lauzardo, Sandra Rivera-Gutierrez
Rok vydání: 2018
Předmět:
Bacterial Diseases
Male
0301 basic medicine
Inertia
Extensively Drug-Resistant Tuberculosis
lcsh:Medicine
Geographical locations
Tuberculosis
Multidrug-Resistant
Medicine and Health Sciences
lcsh:Science
Phylogeny
Genetics
Multidisciplinary
Geography
Multi-Drug-Resistant Tuberculosis
Physics
Multi-drug-resistant tuberculosis
Classical Mechanics
Bacterial Typing Techniques
Actinobacteria
Phylogeography
Infectious Diseases
Biogeography
Physical Sciences
Genetic structure
Female
Research Article
Adult
Tuberculosis
030106 microbiology
Biology
Microbiology
Mycobacterium tuberculosis
Motion
Young Adult
03 medical and health sciences
Microbial Control
Drug Resistance
Bacterial
Genetic variation
medicine
Humans
Multidrug-Resistant Mycobacterium tuberculosis
Mexico
Pharmacology
Evolutionary Biology
Genetic diversity
Bacteria
Population Biology
lcsh:R
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Genetic Variation
Extensively drug-resistant tuberculosis
Tropical Diseases
medicine.disease
biology.organism_classification
Cross-Sectional Studies
Antibiotic Resistance
North America
Earth Sciences
lcsh:Q
Antimicrobial Resistance
People and places
Population Genetics
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 3, p e0193626 (2018)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0193626
Popis: Background Mexico is one of the most important contributors of drug and multidrug-resistant tuberculosis in Latin America; however, knowledge of the genetic diversity of drug-resistant tuberculosis isolates is limited. Methods In this study, the genetic structure of 112 Mycobacterium tuberculosis strains from the southeastern Mexico was determined by spoligotyping and 24-loci MIRU-VNTRs. Findings The results show eight major lineages, the most of which was T1 (24%), followed by LAM (16%) and H (15%). A total of 29 (25%) isolates were identified as orphan. The most abundant SITs were SIT53/T1 and SIT42/LAM9 with 10 isolates each and SIT50/H3 with eight isolates. Fifty-two spoligotype patterns, twenty-seven clusters and ten clonal complexes were observed, demonstrating an important genetic diversity of drug and multidrug-resistant tuberculosis isolates in circulation and transmission level of these aggravated forms of tuberculosis. Being defined as orphan or as part of an orphan cluster, was a risk factor for multidrug resistant-tuberculosis (OR 2.5, IC 1.05–5.86 and OR 3.3, IC 1–11.03, respectively). Multiple correspondence analyses showed association of some clusters and SITs with specific geographical locations. Conclusions Our study provides one of the most detailed description of the genetic structure of drug and multidrug-resistant tuberculosis strains in southeast Mexico, establishing for the first time a baseline of the genotypes observed in resistant isolates circulating, however further studies are required to better elucidate the genetic structure of tuberculosis in region and the factors that could be participating in their dispersion.
Databáze: OpenAIRE
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