The interactions between CdTe quantum dots and proteins: understanding nano-bio interface
| Autor: | Harish M. Gholap, Shreeram Joglekar, Anup Kale, Prashant S. Alegaonkar |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Nanoparticle
Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Fluorescence spectroscopy Protein structure lcsh:TA401-492 chemistry.chemical_classification Quenching (fluorescence) Chemistry Biomolecule quantum dot electrostatic interactions 021001 nanoscience & nanotechnology Binding constant 0104 chemical sciences Chemical engineering Quantum dot Stern-Volmer nano-bio interface lcsh:Materials of engineering and construction. Mechanics of materials fluorescence protein 0210 nano-technology Macromolecule |
| Zdroj: | AIMS Materials Science, Vol 4, Iss 1, Pp 209-222 (2017) |
| ISSN: | 2372-0484 |
| DOI: | 10.3934/matersci.2017.1.209 |
| Popis: | Despite remarkable developments in the nanoscience, relatively little is known about the physical (electrostatic) interactions of nanoparticles with bio macromolecules. These interactions can influence the properties of both nanoparticles and the bio-macromolecules. Understanding this bio-interface is a prerequisite to utilize both nanoparticles and biomolecules for bioengineering. In this study, luminescent, water soluble CdTe quantum dots (QDs) capped with mercaptopropionic acid (MPA) were synthesized by organometallic method and then interaction between nanoparticles (QDs) and three different types of proteins (BSA, Lysozyme and Hemoglobin) were investigated by fluorescence spectroscopy at pH= 7.4. Based on fluorescence quenching results, Stern-Volmer quenching constant (Ksv), binding constant (Kq) and binding sites (n) for proteins were calculated. The results show that protein structure (e.g.,globular, metalloprotein, etc.) has a significant role in Protein-Quantum dots interactions and each type of protein influence physicochemical properties of Quantum dots differently. |
| Databáze: | OpenAIRE |
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