Regulation of Cardiac Stress Signaling by Protein Kinase D1
| Autor: | Eric N. Olson, Rick B. Vega, Philip J. Papst, Brooke C. Harrison, John McAnally, Timothy A. McKinsey, Mi Sung Kim, Rhonda S Bassel-Duby, Eva van Rooij, James A. Richardson, Craig F. Plato |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Heart Ventricles Cardiomyopathy Rats Inbred WF Mice Transgenic Biology urologic and male genital diseases Models Biological Rats Sprague-Dawley Mice Stress Physiological Catalytic Domain Rats Inbred SHR Internal medicine Chlorocebus aethiops medicine Animals Myocyte Myocytes Cardiac RNA Small Interfering Molecular Biology Cells Cultured Protein Kinase C Protein kinase C G protein-coupled receptor Histone deacetylase 5 Articles Cell Biology Cardiomyopathy Hypertrophic medicine.disease female genital diseases and pregnancy complications Rats Cell biology Enzyme Activation Isoenzymes Endocrinology Animals Newborn Gene Expression Regulation COS Cells Phosphorylation Protein kinase D1 Signal transduction Protein Kinases Signal Transduction |
| Zdroj: | Molecular and Cellular Biology. 26:3875-3888 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.26.10.3875-3888.2006 |
| Popis: | In response to pathological stresses such as hypertension or myocardial infarction, the heart undergoes a remodeling process that is associated with myocyte hypertrophy, myocyte death, and fibrosis. Histone deacetylase 5 (HDAC5) is a transcriptional repressor of cardiac remodeling that is subject to phosphorylation-dependent neutralization in response to stress signaling. Recent studies have suggested a role for protein kinase C (PKC) and its downstream effector, protein kinase D1 (PKD1), in the control of HDAC5 phosphorylation. While PKCs are well-documented regulators of cardiac signaling, the function of PKD1 in heart muscle remains unclear. Here, we demonstrate that PKD1 catalytic activity is stimulated in cardiac myocytes by diverse hypertrophic agonists that signal through G protein-coupled receptors (GPCRs) and Rho GTPases. PKD1 activation in cardiomyocytes occurs through PKC-dependent and -independent mechanisms. In vivo, cardiac PKD1 is activated in multiple rodent models of pathological cardiac remodeling. PKD1 activation correlates with phosphorylation-dependent nuclear export of HDAC5, and reduction of endogenous PKD1 expression with small interfering RNA suppresses HDAC5 shuttling and associated cardiomyocyte growth. Conversely, ectopic overexpression of constitutively active PKD1 in mouse heart leads to dilated cardiomyopathy. These findings support a role for PKD1 in the control of pathological remodeling of the heart via its ability to phosphorylate and neutralize HDAC5. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|