Evaluation of cell lines and immunofluorescence and plaque assay procedures for quantifying reoviruses in sewage
| Autor: | R S Spendlove, D N Ridinger, D B George, J C Roth, B B Barnett |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
viruses
Cell Fluorescent Antibody Technique Reoviridae Kidney Immunofluorescence Applied Microbiology and Biotechnology Cell Line medicine Animals Humans Virus quantification Sewage Ecology biology medicine.diagnostic_test urogenital system Embryo biochemical phenomena metabolism and nutrition Cell Transformation Viral Embryo Mammalian biology.organism_classification Macaca mulatta Embryonic stem cell Virology Molecular biology Intestines medicine.anatomical_structure Cell culture Cattle Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 43:740-746 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Twelve continuous cell lines were tested to determine their sensitivity to reovirus types 1, 2, and 3 isolated from sewage. Madin-Darby bovine kidney (MDBK), rhesus monkey kidney (LLC-MK2), and human embryonic intestinal (intestinal 407) cells were most sensitive, respectively. In a similar study, MDBK cells were more sensitive than LLC-MK2 and Buffalo green monkey kidney (BGM) cells to sewage-isolated, protamine-precipitated reoviruses which had not been serotyped and had no previous cell contact. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count and plaque assay procedures. The immunofluorescence assay is more sensitive and more rapid than the plaque assay. Reoviruses in excess of 10(4)/liter of raw sewage were detected by the immunofluorescent cell count assay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|