Evaluation of anti-diabetic and anti-tumoral activities of bioactive compounds from Phoenix dactylifera L's leaf: In vitro and in vivo approach
| Autor: | Mohamed Bouaziz, Mohamed Makni, Naziha Marrakchi, Bassem Khemakhem, M Chakroun, Hazem Ben Mabrouk, Noureddine Drira, Hanen El Abed, Hafedh Mejdoub |
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| Přispěvatelé: | Faculté des Sciences de Sfax, Université de Sfax - University of Sfax, University of Gabes, Laboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules (LR11IPT08), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), École Nationale d'Ingénieurs de Sfax | National School of Engineers of Sfax (ENIS), The major part of this work was accomplished at the Faculty of Sciences of Sfax. The authors would like to thank Prof. Monique Simmonds from Jodrell Laboratory, Kew Gardens UK, for her help with LC–MS/MS analysis. Special thanks are also go to Pr. Hafedh Bejaoui, from the English department at the Faculty of Science of Sfax and to Sana Chakroun (Rudolf-Bultmann Str. 4. 35039 Marburg) for carefully proofreading and polishing the language of the present paper, to Zaineb Kammoun (Sfax Faculty of Science) for her invaluable assistance in this project. |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male MESH: Polyphenols/pharmacology MESH: Plant Extracts/pharmacology [SDV]Life Sciences [q-bio] Flavonoid Phytochemicals Pharmacology MESH: Hyperglycemia/complications MESH: Plant Leaves/chemistry Mice Oral administration Tandem Mass Spectrometry inhibitors MESH: Hypoglycemic Agents/pharmacology MESH: Phytochemicals/pharmacology MESH: Animals α-Glucosidase and α-Amylase Acarbose chemistry.chemical_classification Cell Death MESH: Antineoplastic Agents/pharmacology MESH: Polyphenols/therapeutic use Phoeniceae 04 agricultural and veterinary sciences General Medicine MESH: Hyperglycemia/drug therapy Postprandial Period 040401 food science Postprandial Biochemistry MESH: Phytotherapy MESH: Hypoglycemic Agents/therapeutic use MESH: Postprandial Period MESH: Antineoplastic Agents/therapeutic use medicine.drug LC–MS/MS analysis MESH: Phoeniceae/chemistry MESH: Cell Line Tumor MESH: Phytochemicals/therapeutic use MESH: Diabetes Mellitus Experimental/complications MESH: Diabetes Mellitus Experimental/drug therapy Antineoplastic Agents MESH: alpha-Amylases/metabolism Diabetes Mellitus Experimental 03 medical and health sciences 0404 agricultural biotechnology In vivo MESH: Enzyme Assays Diabetes mellitus Cell Line Tumor medicine [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Animals Humans Hypoglycemic Agents MTT assay IC50 MESH: Mice Enzyme Assays MESH: Plant Extracts/therapeutic use MESH: Humans Cytotoxic activity Plant Extracts MESH: Tandem Mass Spectrometry Polyphenols alpha-Glucosidases medicine.disease IGR-39 cancer cell lines MESH: Diabetes Mellitus Type 2/drug therapy Phoenix dactylifera L.’s leaves MESH: Male [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy MESH: Cell Death/drug effects MESH: Diabetes Mellitus Type 2/complications Plant Leaves 030104 developmental biology Antidiabetic activity chemistry Diabetes Mellitus Type 2 Hyperglycemia alpha-Amylases MESH: alpha-Glucosidases/metabolism MESH: Chromatography Liquid Chromatography Liquid Phytotherapy |
| Zdroj: | Biomedicine and Pharmacotherapy Biomedicine and Pharmacotherapy, Elsevier Masson, 2016, 84, pp.415-422. ⟨10.1016/j.biopha.2016.09.062⟩ |
| ISSN: | 1950-6007 0753-3322 |
| DOI: | 10.1016/j.biopha.2016.09.062⟩ |
| Popis: | International audience; Among various chronic disorders, cancer and diabetes mellitus are the most common disorders. This study was designed to evaluate the effectiveness of hydroalcoholic extract of Phoenix dactylifera L. leaves (HEPdL) in animal models of type II diabetes in vitro/in vivo and in a human melanoma-derived cell line (IGR-39). A liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis was also performed to determine the amount of phenolic and flavonoid compounds in this plant. The physicochemical results by LC–MS/MS analysis of HEPdL showed the presence of 10 phenolic compounds. The in vitro study showed that the extract exhibited a more specific and potent inhibitor of α-glucosidase than α-amylase with an IC50 value of 20 ± 1 μg/mL and 30 ± 0.8 μg/mL, respectively. More importantly, the in vivo study of the postprandial hyperglycemia activity with (20 mg/kg) of HEPdL showed a decrease in plasma glucose levels after 60 min in resemblance to the glucor (acarbose) (50 mg/kg) effect. The oral administration of HEPdL (20 mg/kg) in alloxan-induced diabetic mices for 28 days showed a more significant anti-diabetic activity than that of the drug (50 mg/kg). Moreover, cytotoxicity effects of HEPdL in IGR-39 cancer cell lines were tested by MTT assay. This extract was effective in inhibiting cancer cells growth (IGR-39) at dose 35 and 75 μg/mL. These results confirm ethnopharmacological significance of the plant and could be taken further for the development of an effective pharmaceutical drug against diabetes and cancer |
| Databáze: | OpenAIRE |
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