Automated in situ detection (AISD) of biomolecules

Autor:	Gerd Gehrke, Jörg Schlossherr, Hady Ibrahim, Martin Gajewski, Heinrich Gausepohl, Günter Plickert
Rok vydání:	2016
Předmět:	chemistry.chemical_classification In situ Biomolecule In situ hybridization Biology Molecular biology Biological materials Large sample Handling system chemistry Expression analysis Genetics Developmental Biology Biomedical engineering
Zdroj:	Development genes and evolution. 207(5)
ISSN:	1432-041X
Popis:	In order to facilitate in situ detection of biomolecules in large sample series the processing of whole-mount specimens has been automated. A freely programmable liquid handling system is described by which embryos or similar biological materials are processed. Possible applications include in situ hybridization (ISH), immunocytochemistry (ICC) or reporter gene assays. Process times required for the preparation of whole-mount in situ hybridizations in Drosophila, Xenopus, Gallus and in hydroids were - in part - significantly reduced as compared with manual processing. Application of automated in situ detection (AISD) in random screening is demonstrated in hydroids. Potential further applications are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::637a66446be83d5769096d4211c5b922 https://pubmed.ncbi.nlm.nih.gov/27747434 Zobrazit plný text záznamu Full text from SpringerLink