Myocardial dna strand breaks are detected in biopsy tissues from patients with dilated cardiomyopathy
| Autor: | Akihiko Muraishi, Kyo Adachi, Yukihiko Seki, Hisashi Kai, Tsutomu Imaizumi, Mamiko Kai |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Cardiomyopathy Dilated Male Pathology medicine.medical_specialty Myocarditis Biopsy Apoptosis Interstitial cell Pathogenesis Fibrosis medicine Humans Myocyte cardiovascular diseases TUNEL assay medicine.diagnostic_test business.industry Myocardium Dilated cardiomyopathy Articles General Medicine Middle Aged musculoskeletal system medicine.disease Microscopy Electron Case-Control Studies cardiovascular system Female Cardiology and Cardiovascular Medicine business DNA Damage |
| Zdroj: | Clinical Cardiology. 21:591-596 |
| ISSN: | 1932-8737 0160-9289 |
| DOI: | 10.1002/clc.4960210811 |
| Popis: | Background: Progressive damage of cardiomyocytes with interstitial and replacement fibrosis accompanied by less inflammatory cell infiltration is observed in patients with dilated cardiomyopathy (DCM), suggesting some other mechanisms rather than necrotic cell death. Hypothesis: The aim of this study was to assess the possible involvement of apoptotic process in the pathogenesis of DCM and myocarditis. Methods: Endomyocardial biopsy was performed in patients with DCM (n = 9). myocarditis (n = 4), or atypical chest pain syndrome (as controls; n = 5). The TUNEL method was used for in situ detection of oligonucleosomal DNA strand breaks. Results: The TUNEL-positive cells were observed in three of nine patients with DCM and in all four with myocarditis, but in none of the controls. The TUNEL-positive nuclei were observed exclusively in cardiomyocytes in DCM, whereas in myocarditis they were detected mainly in interstitial cells and in a few myocytes. In DCM, interstitial fibrosis was greater in the TUNEL-positive than in TUNEL-negative patients (p |
| Databáze: | OpenAIRE |
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