Role of oxygen and nitrogen species in experimental uveitis: anti-inflammatory activity of the synthetic antioxidant ebselen
| Autor: | Belén Romero, Nuria Marín, Antonio Rodríguez-Galietero, Manuel Díaz-Llopis, Francisco Bosch-Morell, Francisco J. Romero, Siv Johnsen-Soriano, Joaquín Romá |
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| Rok vydání: | 2002 |
| Předmět: |
Azoles
Free Radicals Chlorpromazine Anti-Inflammatory Agents Pharmacology Isoindoles medicine.disease_cause Biochemistry Antioxidants Superoxide dismutase Uveitis chemistry.chemical_compound Physiology (medical) Malondialdehyde Organoselenium Compounds medicine Animals Enzyme Inhibitors Reactive nitrogen species chemistry.chemical_classification Reactive oxygen species biology Chemistry Superoxide Ebselen Superoxide Dismutase Glutathione peroxidase Reactive Nitrogen Species Disease Models Animal NG-Nitroarginine Methyl Ester biology.protein Lipid Peroxidation Rabbits Nitric Oxide Synthase Reactive Oxygen Species Oxidative stress |
| Zdroj: | Free radical biologymedicine. 33(5) |
| ISSN: | 0891-5849 |
| Popis: | This study was aimed at examining the role of oxygen and nitrogen reactive species in a model of experimental uveitis upon intravitreal injection of bacterial endotoxin to albino New Zealand rabbits. The inflammatory response was evaluated in terms of: (i) the integrity of the blood aqueous barrier (protein and cell content in samples of aqueous humor), (ii) histopathological changes of the eyes, (iii) clinical evaluation (with a score index based on clinical symptoms), and (iv) the concentration of malondialdehyde (MDA), in aqueous humor, as a marker of oxidative stress. Betamethasone was used as reference treatment, superoxide dismutase as quencher of superoxide anion, L-N(G)-nitro-L-arginine-methyl-esther (L-NAME) and chlorpromazine as nitric oxide synthase inhibitors, and ebselen, a glutathione peroxidase mimic, as peroxynitrite reductant. All the substances were injected subconjunctivally to the rabbits immediately after the intravitreal endotoxin injection. Ebselen was the only treatment able to decrease MDA concentration to control values, exerting an effect similar to that elicited by L-NAME on the rest of the parameters tested. The data presented render ebselen a notable choice for the treatment of uveitis, with implications for clinical trials. |
| Databáze: | OpenAIRE |
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