Survey of community-associated-methicillin-Resistant Staphylococcus aureus in Slovenia: identification of community-associated and livestock-associated clones
| Autor: | H. Ribic, N. Švent-Kučina, Ž. Petrovič, I. Grmek-Kosnik, M. Mueller-Premru, Sandra Janežič, Maja Rupnik, U. Dermota, Irena Zdovc |
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| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Methicillin-Resistant Staphylococcus aureus Livestock Bacterial Toxins Slovenia Erythromycin Exotoxins Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology Bacterial Proteins Ciprofloxacin Leukocidins Drug Resistance Multiple Bacterial medicine Animals Penicillin-Binding Proteins Cefoxitin Cloning Molecular Microbial Viability SCCmec Clindamycin General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Anti-Bacterial Agents Infectious Diseases Staphylococcus aureus Multilocus sequence typing Gentamicins medicine.drug |
| Zdroj: | International journal of medical microbiology : IJMM. 305(6) |
| ISSN: | 1618-0607 |
| Popis: | The epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in Slovenia is poorly documented. The aim of this study was to investigate susceptibility patterns, virulence gene profile and clonality among MRSA isolates with positive screened resistance phenotype for CA-MRSA collected from patients in Slovenia, from January 2010 to December 2010. We included only MRSA isolates that were resistant to cefoxitin and oxacillin, and susceptible to at least two of the following four antibiotics: ciprofloxacin, erythromycin, clindamycin or gentamicin (presumptive CA-MRSA). Altogether 151 isolates fulfilled our screening phenotypic definition, 126 MRSA isolates were classified as CA-MRSA and 25 as HA-MRSA. Thirty-six per cent of them were resistant to ciprofloxacin, 24% to clindamycin, 33% to erythromycin and 13% to gentamicin. The mecA gene was detected in 150 isolates, while the mecC gene only in 1 isolate. The MRSA isolates were classified to 19 different clones. The most prevalent sequence types were ST5 (26.4%), ST45 (25.2%), ST22 (10.6%), ST398 (9.9%), ST8 (5.9%), ST7 (4.6%), ST1 (3.9%), ST152/377 (3.3%), ST228 (2.6%) and ST2883 (1.3%). The ST6, ST9, ST30, ST72, ST88, ST111, ST130, ST225 and ST772 were identified sporadically. The Panton-Valentine leukocidin (PVL) gene was detected in 13 (8.6%) isolates that belonged to ST5, ST7, ST8, ST22, ST72, ST88, ST 152/377 and ST772. Our results show high variability of CA-MRSA circulating in Slovenia and also the presence of LA-MRSA clones. |
| Databáze: | OpenAIRE |
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