Simultaneous determination of four neuroprotective compounds of Tilia amurensis by high performance liquid chromatography coupled with diode array detector
| Autor: | Choong Je Ma, Jiwoo Lee, Bo-Ra Yun, Jin Bae Weon, Bohyung Lee, Min Rye Eom |
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| Rok vydání: | 2013 |
| Předmět: |
Detection limit
Chromatography Tilia amurensis Calibration curve Analytical chemistry Pharmaceutical Science High-performance liquid chromatography chemistry.chemical_compound chemistry Chromatography detector Scopoletin Drug Discovery Trifluoroacetic acid Gradient elution Original Article simultaneous determination quality control High performance liquid chromatography/diode array detector |
| Zdroj: | Pharmacognosy Magazine |
| ISSN: | 0973-1296 |
| Popis: | Background: Tilia amurensis consists of various compounds, such as flavonoids and terpenoids. Objective: A simple and reliable high performance liquid chromatography (HPLC) coupled with the diode array detector (DAD) method has been established for simultaneous determination of epicatechin, nudiposide, lyoniside, and scopoletin isolated from Tilia amurensis. Materials and Methods: Optimum separations were obtained with a SHISEIDO C18 column by gradient eluton, with 0.1% Trifluoroacetic acid (TFA) water-methanol as the mobile phase. The gradient elution system was completed within 40 minutes. The flow rate and detection wavelength were 1 mL/minute, 205 nm, 250 nm, and 280 nm, respectively. Results: Validation of the analytical method was evaluated by linearity, precision, and the accuracy test. The calibration curve was linear over the established range with R2 > 0.997. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01-15.20 μg/mL and 0.03-46.06 μg/mL. The method exhibited an intraday and interday precision range of 96.25-105.66% and 93.52-109.92%, respectively (RSD |
| Databáze: | OpenAIRE |
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