Oriental Hornet (Vespa orientalis) Larval Extracts Induce Antiproliferative, Antioxidant, Anti-Inflammatory, and Anti-Migratory Effects on MCF7 Cells

Autor: Mohammed A. El-Magd, Omar Bahattab, Osama Al-Amer, Amina M. G. Zedan, Atif Abdulwahab A. Oyouni, Yousef M Hawsawi, Samah K Nasr Eldeen, Mohamed I. Sakran
Rok vydání: 2021
Předmět:
Naringenin
antioxidant
Antioxidant
medicine.medical_treatment
Wasps
Anti-Inflammatory Agents
Pharmaceutical Science
Pharmacology
Resveratrol
Antioxidants
Analytical Chemistry
chemistry.chemical_compound
QD241-441
0302 clinical medicine
Cell Movement
Drug Discovery
Homeostasis
anti-inflammatory
0303 health sciences
Chemistry
Rosmarinic acid
MCF7
Vespa orientalis
apoptosis
Free Radical Scavengers
Chemistry (miscellaneous)
Larva
030220 oncology & carcinogenesis
Flavanones
MCF-7 Cells
Molecular Medicine
Oxidation-Reduction
medicine.drug_class
Antineoplastic Agents
Depsides
Article
Anti-inflammatory
03 medical and health sciences
Picrates
Downregulation and upregulation
Cell Line
Tumor
medicine
Animals
Humans
Viability assay
Physical and Theoretical Chemistry
Cell Proliferation
030304 developmental biology
Inflammation
Wound Healing
Biphenyl Compounds
fungi
Organic Chemistry
Cinnamates
Apoptosis
anti-migration
Zdroj: Molecules, Vol 26, Iss 3303, p 3303 (2021)
Molecules
Volume 26
Issue 11
ISSN: 1420-3049
Popis: The use of insects as a feasible and useful natural product resource is a novel and promising option in alternative medicine. Several components from insects and their larvae have been found to inhibit molecular pathways in different stages of cancer. This study aimed to analyze the effect of aqueous and alcoholic extracts of Vespa orientalis larvae on breast cancer MCF7 cells and investigate the underlying mechanisms. Our results showed that individual treatment with 5% aqueous or alcoholic larval extract inhibited MCF7 proliferation but had no cytotoxic effect on normal Vero cells. The anticancer effect was mediated through (1) induction of apoptosis, as indicated by increased expression of apoptotic genes (Bax, caspase3, and p53) and decreased expression of the anti-apoptotic gene Bcl2
(2) suppression of intracellular reactive oxygen species
(3) elevation of antioxidant enzymes (CAT, SOD, and GPx) and upregulation of the antioxidant regulator Nrf2 and its downstream target HO-1
(4) inhibition of migration as revealed by in vitro wound healing assay and downregulation of the migration-related gene MMP9 and upregulation of the anti-migratory gene TIMP1
and (5) downregulation of inflammation-related genes (NFκB and IL8). The aqueous extract exhibited the best anticancer effect with higher antioxidant activities but lower anti-inflammatory properties than the alcoholic extract. HPLC analysis revealed the presence of several flavonoids and phenolic compounds with highest concentrations for resveratrol and naringenin in aqueous extract and rosmarinic acid in alcoholic extract. This is the first report to explain the intracellular pathway by which flavonoids and phenolic compounds-rich extracts of Vespa orientalis larvae could induce MCF7 cell viability loss through the initiation of apoptosis, activation of antioxidants, and inhibition of migration and inflammation. Therefore, these extracts could be used as adjuvants for anticancer drugs and as antioxidant and anti-inflammatory agents.
Databáze: OpenAIRE
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