Rapid flow cytometric assay for the assessment of natural killer cell activity
| Autor: | Gary Gusewitch, Sandra L. Nehlsen-Cannarella, Lauralynn K. Lebeck, Leh Chang, Douglas B. W. Chritton, Judith C. Folz |
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| Rok vydání: | 1993 |
| Předmět: |
Adult
Immunology Cell Biology In Vitro Techniques Stain Flow cytometry Natural killer cell medicine Tumor Cells Cultured Immunology and Allergy Humans Cytotoxicity Fluorescent Dyes medicine.diagnostic_test Effector Carbocyanines Cytotoxicity Tests Immunologic Flow Cytometry Fluorescence Molecular biology Chromium Radioisotopes Killer Cells Natural Kinetics medicine.anatomical_structure Evaluation Studies as Topic K562 cells |
| Zdroj: | Journal of immunological methods. 166(1) |
| ISSN: | 0022-1759 |
| Popis: | A new assay using flow cytometry has been established to assess natural killer (NK) lytic activity with common bench top instrumentation. This assay uses a cyanine membrane dye to stain live K562 target cells and an iodide nuclear dye to evaluate dead cells, and provides a method of reliably separating target and effector cell populations. Effector cells remain unstained (fluorescent negative) throughout the procedure. The damaged pre-labeled targets appear doubly stained as their membranes become permeable to the nuclear stain during incubation. Percent cytotoxicity of various effector:target cell ratios is discerned using flow cytometric analysis after a 2 h incubation in this new assay, as compared to 4 h with the 51 Cr-release ‘gold standard’ assay for cell-mediated cytotoxicity. Comparisons of normal individuals tested in parallel with the fluorescent dyes and the 51 Cr-release assay have shown direct correlations. This new two-color flow cytometric technique has proven to be uncomplicated and reproducible when used in the clinical setting. |
| Databáze: | OpenAIRE |
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