Pro-inflammatory agents accumulate during donor liver cold preservation: a study on increased adhesion molecule expression and abrogation by curcumin in cultured endothelial cells
| Autor: | Patrice Butler, Barry Fuller, Sas Dijk, Brian R. Davidson, Van Hoang |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Curcumin
Intercellular Adhesion Molecule-1 Cold storage Biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound medicine Humans Northern blot Cells Cultured Cryopreservation Cell adhesion molecule Anti-Inflammatory Agents Non-Steroidal Soluble cell adhesion molecules General Medicine medicine.disease Tissue Donors Cell biology Transplantation Liver chemistry Endothelium Vascular E-Selectin General Agricultural and Biological Sciences Reperfusion injury |
| Zdroj: | Cryobiology. 46:284-288 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/s0011-2240(03)00027-0 |
| Popis: | Cold hypoxia and re-oxygenation during organ preservation leads to a variety of stresses, including expression of adhesion molecules, which can promote inflammatory responses and compromised function. These studies were undertaken to investigate the pro-inflammatory activities of factors present in the vascular bed at the end of cold storage in livers during clinical hepatic transplantation. Effluent perfusate (termed pro-inflammatory vascular effluent—PVE) washed out at the end of cold storage, was collected from 10 donor livers, and used in a bio-assay with cultured endothelial cells exposed to cold hypoxia and re-oxygenation. Adhesion molecule expressions for intercellular adhesion molecule 1 (ICAM-1) and E-selectin were measured using molecular techniques and Northern blot analyses. Cells exposed to PVE during warming showed up-regulation of both ICAM-1 and E-selectin (p |
| Databáze: | OpenAIRE |
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