Integrating insulin into single-step culture medium regulates human embryo development in vitro
| Autor: | Mohamed Abdel-Rahman, Hazem Abdelghafar, Yasmin Magdi, Mohamed Sabry, Mohamed Fawzy, Mohamed Nour, Eman Roshdy |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Time Factors Adolescent Pregnancy Rate medicine.medical_treatment Embryonic Development Oocyte Retrieval Biology Intracytoplasmic sperm injection Cryopreservation Andrology Embryo Culture Techniques 03 medical and health sciences Young Adult 0302 clinical medicine Pregnancy medicine Humans Insulin Blastocyst Prospective Studies Sperm Injections Intracytoplasmic Gynecology 030219 obstetrics & reproductive medicine Obstetrics and Gynecology Embryo culture Embryo Fertility Agents Female Embryo Transfer Embryo transfer Culture Media 030104 developmental biology medicine.anatomical_structure Fertility Treatment Outcome Reproductive Medicine Infertility Pregnancy Twin Egypt Female Embryo quality |
| Zdroj: | Fertility and sterility. 107(2) |
| ISSN: | 1556-5653 |
| Popis: | Objective To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development. Design Comparative study. Setting Two private centers. Patient(s) The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients. Intervention(s) Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium. Women were split to receive their embryos from insulin-supplemented or control medium. Main Outcome Measure(s) Clinical pregnancy rate. Result(s) There were significantly higher rates of clinical, ongoing, and twin pregnancies in the insulin-supplemented arm than in the control arm. On day 3, embryo quality and compaction were higher in insulin-supplemented medium. On day 5, insulin supplementation showed higher rates of blastocyst formation, quality, and cryopreservation. Conclusion(s) Insulin supplementation of single-step embryo culture medium from day 0 through day 5 or 6 improved clinical pregnancy rate and human embryo development. However, these findings need further confirmation through a multicenter randomized controlled trial that may include other patient populations and different culture media. |
| Databáze: | OpenAIRE |
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