Comparative Inhibitory Effects of Antigen and Antibody in the Staphylococcal Enterotoxin Solid-Phase Radioimmunoassay System
| Autor: | Howard M. Johnson, Joann A. Bukovic, James T. Peeler |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Staphylococcus
Radioimmunoassay Enterotoxin Inhibitory postsynaptic potential medicine.disease_cause Binding Competitive Models Biological General Biochemistry Genetics and Molecular Biology Antigen-Antibody Reactions Iodine Radioisotopes Enterotoxins Antigen Solid phase radioimmunoassay medicine Animals General Pharmacology Toxicology and Pharmaceutics Binding site Food Microbiology and Toxicology General Immunology and Microbiology biology Chemistry Immune Sera Chloramines General Medicine Antibodies Bacterial Molecular biology biology.protein Polystyrenes Binding Sites Antibody Rabbits Antibody |
| Zdroj: | Applied Microbiology. 29:765-768 |
| ISSN: | 0003-6919 |
| DOI: | 10.1128/am.29.6.765-768.1975 |
| Popis: | A solid-phase radioimmunoassay employing 125 I-labeled enterotoxins and polystyrene tubes coated with specific antibody has been developed for assaying the relative concentrations of antibodies to staphylococcal enterotoxins A and B. Competitive binding occurs between tube-bound antibody and free antibody for binding sites on 125 I-labeled enterotoxin. The sensitivity of the system is affected by the amount of antibody on the walls of the tubes, the concentration of 125 I-labeled enterotoxin added to the system, and probably by the relative binding affinities of the bound and unbound antibodies. Antibody, 0.01 to 0.07 μg/ml, inhibited the uptake of 125 I-labeled enterotoxin by 20%. Both the antibody and antigen solid-phase radioimmunoassay inhibition systems can be appropriately represented by either of the following two models: Log e ( Y /1 - Y ) = α 0 + α 1 Log e X and Log e Y = β 0 + β 1 Log e X , where Y is bound activity, X is antigen or antibody concentration for inhibition, and α 0 , α 1 , β 0 , and β 1 are regression coefficients. Estimates from the first model were slightly more precise for the antibody system, whereas the reverse was true for the antigen system. |
| Databáze: | OpenAIRE |
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