Further evidence on the role of heparan sulfate as protamine acceptor during the decondensation of human spermatozoa

Autor: Consuelo Baldini, Lucrecia Calvo, Eleonora Regueira, Marina Romanato, Mónica S. Cameo, Juan Carlos Calvo
Rok vydání: 2005
Předmět:
Male
Time Factors
Protamine
Heparan sulfate
Sodium Chloride
heparin
chemistry.chemical_compound
immunocytochemistry
Urea
Microscopy
Phase-Contrast
Protamines
glutathione
Polyacrylamide gel electrophoresis
Glycosaminoglycans
biology
Rehabilitation
article
Obstetrics and Gynecology
Heparin
Glutathione
Immunohistochemistry
Spermatozoa
Chromatin
Biochemistry
glutaraldehyde
Electrophoresis
Polyacrylamide Gel
spermatozoon capacitation
medicine.drug
endocrine system
in vitro study
Protamine sulfate
chromatin condensation
Semen
semen analysis
Fixatives
cell isolation
drug activity
male
protein secretion
glycosaminoglycan
medicine
Humans
carbohydrate analysis
Sperm nuclear decondensation
human
Mercaptoethanol
Cell Nucleus
spermatozoon density
urogenital system
human cell
Sperm
Kinetics
Reproductive Medicine
chemistry
Glutaral
phase contrast microscopy
protein analysis
protein isolation
biology.protein
Heparitin Sulfate
Sulfur
polyacrylamide gel electrophoresis
Zdroj: Hum. Reprod. 2005;20(10):2784-2789
Biblioteca Digital (UBA-FCEN)
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
ISSN: 1460-2350
0268-1161
DOI: 10.1093/humrep/dei124
Popis: Background: Human spermatozoa decondense in vitro upon exposure to heparin and glutathione. Glutathione is also the disulfide bond reducer in vivo, and heparan sulfate, a functional analogue of heparin, has been proposed as the protamine acceptor. The aim of this study was to evaluate the decondensing ability of chemically modified heparins and different glycosaminoglycans (GAGs) on isolated sperm nuclei in vitro, and to analyse the possible role of different GAGs as protamine acceptors. Methods: Capacitated spermatozoa and isolated sperm nuclei from normospermic semen samples were decondensed in the presence of heparin (or its equivalent) and glutathione. After fixation with glutaraldehyde, the percentage of decondensed spermatozoa and nuclei was determined under phase-contrast. Proteins were extracted from sperm nuclei previously incubated in the presence of gluhathione and different GAGs by incubation with urea-β-meracptoethanol-NaCl, and analysed by acid polyacrylamide gel electrophoresis. Results: The ability of desulfated heparins and other GAGs to decondense isolated nuclei mirrored exactly the decondensation of capacitated spermatozoa, the only difference being the level of maximum decondensation achieved. Heparan sulfate and heparin, but not other GAGs, were able to release protamines from sperm chromatin. Conclusions: Heparan sulfate could be functioning as protamine acceptor in vivo during human sperm nuclear decondensation. © The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. Fil:Romanato, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Regueira, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Databáze: OpenAIRE
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