Quantitative immunocytochemistry of Ca(2+)-Mg2+ ATPase in ameloblasts associated with enamel secretion and maturation in the rat incisor
| Autor: | Arthur R. Hand, D. R. Eisenmann, James L. Borke, M. I. Mednieks, Abd El-Moneim Zaki |
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| Rok vydání: | 1996 |
| Předmět: |
0301 basic medicine
Male Cytoplasm ATPase Calcium pump Immunocytochemistry Calcium-Transporting ATPases Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine stomatognathic system Dental Enamel Proteins Amelogenesis Ameloblasts Animals Humans Dental Enamel Analysis of Variance biology Enamel paint Membrane Proteins 030206 dentistry General Medicine Immunogold labelling Molecular biology Immunohistochemistry Rats Incisor 030104 developmental biology visual_art biology.protein visual_art.visual_art_medium Cytochemistry Ca(2+) Mg(2+)-ATPase Ameloblast Tooth Calcification |
| Zdroj: | Advances in dental research. 10(2) |
| ISSN: | 0895-9374 |
| Popis: | Our previous studies revealed intense membrane-associated labeling for Ca2+-Mg2+ ATPase (Ca2+-pump) in secretory and maturation ameloblasts in the rat incisor, both by enzyme cytochemistry and by immunohistochemical techniques. The purpose of the present study was to map the distribution of Ca2+-pump protein at the cellular and subcellular levels by means of a Ca2+-pump-specific monoclonal antibody and electron microscopic immunogold cytochemistry. Tissue specimens were dissected from secretory, early, and late enamel maturation zones. We quantified results by comparing gold particle densities over ameloblast lateral and distal plasma membrane regions, supranuclear cytoplasm, regions of the ruffled borders, and nuclei. The highest concentration of gold particles was seen over the distal membranes of early-maturation ameloblasts relative to those in late-maturation and secretory stages. Cytoplasmic labeling was less than that of the distal and lateral membranes, and gold particles located over nuclei were considered to be due to non-specific binding. These results are consistent with our earlier findings and suggest a role for the plasma membrane Ca2+-pump in the regulation of calcium availability to mineralizing enamel. |
| Databáze: | OpenAIRE |
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