Antibody mediated fluorescence enhancement of nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC°)
| Autor: | Jörg A. Schenk, Christine Lenz, Anika Andersson, L. Marcus Wilhelmsson, Frank Sellrie |
|---|---|
| Přispěvatelé: | Publica |
| Rok vydání: | 2014 |
| Předmět: |
medicine.drug_class
Stereochemistry Antibody Affinity Enzyme-Linked Immunosorbent Assay Monoclonal antibody Fluorescence Analytical Chemistry chemistry.chemical_compound Antibody Specificity Oxazines medicine Immunoassay Molecular Structure Nucleoside analogue biology medicine.diagnostic_test Chemistry Antibodies Monoclonal Reproducibility of Results DNA Combinatorial chemistry Spectrometry Fluorescence Homogeneous biology.protein Deoxycytidine Antibody medicine.drug |
| Zdroj: | Talanta. 124:67-70 |
| ISSN: | 0039-9140 |
| Popis: | We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC°). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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