Three distinct ribosome assemblies modulated by translation are the building blocks of polysomes
| Autor: | Paolo Bianchini, Alessandro Quattrone, Paola Bernabo, Toma Tebaldi, Cecilia Pederzolli, Lorenzo Lunelli, Robert J.C. Gilbert, Andrea Passerini, Gabriella Viero, Alberto Diaspro |
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| Rok vydání: | 2015 |
| Předmět: |
endocrine system
Messenger FREE PROTEIN-SYNTHESIS Biology Ribosome Article Cell Line 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Polysome Gene expression Protein biosynthesis Humans RNA Messenger Ribosome profiling Research Articles ATOMIC-FORCE MICROSCOPY 030304 developmental biology 0303 health sciences Messenger RNA Tumor RNA Translation (biology) Polyribosomes Protein Biosynthesis Cell Biology DOUBLE-ROW POLYRIBOSOMES Cell biology MESSENGER-RNA TRANSLATION 030217 neurology & neurosurgery |
| Zdroj: | The journal of cell biology (Online) 208 (2015): 581–596. doi:10.1083/jcb.201406040 info:cnr-pdr/source/autori:Viero G.; Lunelli L.; Passerini A.; Bianchini P.; Gilbert R.J.; Bernabo P.; Tebaldi T.; Diaspro A.; Pederzolli C.; Quattrone A./titolo:Three distinct ribosome assemblies modulated by translation are the building blocks of polysomes/doi:10.1083%2Fjcb.201406040/rivista:The journal of cell biology (Online)/anno:2015/pagina_da:581/pagina_a:596/intervallo_pagine:581–596/volume:208 The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Recurrent supra-ribosomal building blocks separated by ribosome-free mRNA regions form polysomes and reflect cellular translational activity. Translation is increasingly recognized as a central control layer of gene expression in eukaryotic cells. The overall organization of mRNA and ribosomes within polysomes, as well as the possible role of this organization in translation are poorly understood. Here we show that polysomes are primarily formed by three distinct classes of ribosome assemblies. We observe that these assemblies can be connected by naked RNA regions of the transcript. We show that the relative proportions of the three classes of ribosome assemblies reflect, and probably dictate, the level of translational activity. These results reveal the existence of recurrent supra-ribosomal building blocks forming polysomes and suggest the presence of unexplored translational controls embedded in the polysome structure. |
| Databáze: | OpenAIRE |
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