Conserved effects and altered trafficking of Cetuximab antibodies conjugated to gold nanoparticles with precise control of their number and orientation
| Autor: | Olivia Tort, Lorena García-Fernández, Eudald Casals, Mathias Brust, Víctor F. Puntes, Ian A. Prior, Julia Lorenzo, Javier Garcia-Pardo |
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| Rok vydání: | 2017 |
| Předmět: |
Receptor recycling
Materials science medicine.drug_class medicine.medical_treatment Cetuximab Metal Nanoparticles Antineoplastic Agents 02 engineering and technology 010402 general chemistry Monoclonal antibody 01 natural sciences Growth factor receptor Cell Line Tumor medicine Humans General Materials Science biology Immunotherapy 021001 nanoscience & nanotechnology In vitro 0104 chemical sciences ErbB Receptors Colloidal gold Immunology biology.protein Biophysics Gold Antibody 0210 nano-technology medicine.drug |
| Zdroj: | Nanoscale. 9:6111-6121 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/c7nr00947j |
| Popis: | Gold nanoparticles (17 nm) have been functionalized with the antiangiogenic monoclonal antibody drug Cetuximab at a well-defined orientation and coverage density of antibodies. Functionalization has been carried out through site-directed chemistry via the selective oxidation of the carbohydrate moiety of antibodies linked to a thiolated hydrazide. A431 tumor cells have been exposed to these conjugates for in vitro evaluation of their effects. In addition to epithelial growth factor receptor blocking, trafficking and signaling alterations were also observed. Thus, the blocking effects of Cetuximab were increased and sustained for a longer time when associated with the nanoparticles. Enhancing antibody therapy effects by decreasing the needed dose and prolonging its effect by avoiding receptor recycling may serve to obtain increased therapeutic benefits for immunotherapy. |
| Databáze: | OpenAIRE |
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