Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry
| Autor: | Rodrigo Ramos Catharino, Júlio César Miné, Cibele Zanardi Esteves, Diogo Noin de Oliveira, Carlos Fernando Odir Rodrigues Melo, Silmara Marques Allegretti, Tatiane Melina Guerreiro, Estela de Oliveira Lima, Rosimeire Nunes de Oliveira |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Ascariosis Late development Mass Spectrometry 03 medical and health sciences Feces 0302 clinical medicine Metabolomics medicine Helminths Animals Humans Ascaris lumbricoides Ascariasis Complex matrix General Veterinary biology General Medicine biology.organism_classification medicine.disease 030104 developmental biology Infectious Diseases Biochemistry Insect Science Parasitic disease Larva Immunology Parasitology Female 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | Parasitology research. 115(11) |
| ISSN: | 1432-1955 |
| Popis: | Ascaris lumbricoides is responsible for a highly disseminated helminth parasitic disease, ascariosis, a relevant parasitosis that responds for great financial burden on the public health system of developing countries. In this work, metabolic fingerprinting using high-resolution mass spectrometry (HRMS) was employed to identify marker molecules from A. lumbricoides in different development stages. We have identified nine biomarkers, such as pheromones and steroidal prohormones in early stages, among other molecules in late development stages, making up four molecules for fertilized eggs, four marker molecules for first larvae (L1) and one marker molecule for third larvae (L3). Therefore, our findings indicate that this approach is suitable for biochemical characterization of A. lumbricoides development stages. Moreover, the straightforward analytical method employed, with almost no sample preparation from a complex matrix (feces) using high-resolution mass spectrometry, suggests that it is possible to seek for an easier and faster way to study animal molding processes. |
| Databáze: | OpenAIRE |
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