Additional file 3 of Obesity and dyslipidemia are associated with partially reversible modifications to DNA hydroxymethylation of apoptosis- and senescence-related genes in swine adipose-derived mesenchymal stem/stromal cells

Autor: Glasstetter, Logan M., Oderinde, Tomiwa S., Mirchandani, Mohit, Rajagopalan, Kamalnath Sankaran, Barsom, Samer H., Thaler, Roman, Siddiqi, Sarosh, Zhu, Xiang-Yang, Tang, Hui, Jordan, Kyra L., Saadiq, Ishran M., van Wijnen, Andre J., Eirin, Alfonso, Lerman, Lilach O.
Rok vydání: 2023
DOI: 10.6084/m9.figshare.23207947.v1
Popis: Additional file 3: Figure S2. Protein expression of differentially hydroxymethylated candidate genes with functions related to apoptosis, senescence, or cell proliferation. A Protein levels were evaluated by Western blotting in swine Lean- and Obese-MSCs, n = 6 each; B quantification revealed decreased levels of the apoptosis-related genes CHOP/DDIT3 and CASP14 in Obese-MSCs compared with Lean-MSCs. The rightmost lane is excluded from quantification of the Western blot for CASP14, due to an obstructive artifact. Displayed Western blot images have been cropped, and group annotations are shown by white or gray horizontal bars above the images. Full-length blots/gels are presented in Figure S3. *p-value ≤ 0.05 vs. Lean-MSCs.
Databáze: OpenAIRE